INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA
文献类型：期刊论文
| 作者 | Cai, Linlin1,2; Zhou, Qiaohong1; He, Feng1; Kong, Lingwei1,2; Hu, Shenghua1; Wu, Zhenbin1 |
| 刊名 | FRESENIUS ENVIRONMENTAL BULLETIN
 |
| 出版日期 | 2012 |
| 卷号 | 21期号:6页码:1357-1364 |
| 关键词 | Microbial community structure culture dependent and independent PCR-DGGE western West Lake |
| ISSN号 | 1018-4619 |
| 通讯作者 | Wu, ZB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Culture-dependent and independent PCR-DGGE methods were used to investigate the genetic diversity of microbial community in the western West Lake, Hangzhou. Water samples (n=7) were collected from the western West Lake in August, 2010. Crude DNA was directly extracted from water samples and mix-culture samples, and then used as the templates for the amplification of 16S rDNA V-3 region. The fingerprinting map of PCR-DGGE, sequencing and phylogenetic analysis all indicated that microbial communities were highly variable in the western West Lake and most microbes existed in very low abundance. The sublake Wuguitan had the highest microbial diversity (represented by 27 DGGE bands). The microbial communities of the physically isolated sub-lakes were similar in the western West Lake while the microbial communities dramatically changed at different sampling sites in the same sublake. The two dominant microorganisms detected by culture-independent PCR-DGGE are closely related to the uncultured bacterium HQ671854.1 and anaerobic bacterium ANA No.40 AB064556.1 while the most abundant microbes were found to be Acinetobacter sp. and Pseudomonas sp. by using culture dependent PCR-DGGE method. |
| 英文摘要 | Culture-dependent and independent PCR-DGGE methods were used to investigate the genetic diversity of microbial community in the western West Lake, Hangzhou. Water samples (n=7) were collected from the western West Lake in August, 2010. Crude DNA was directly extracted from water samples and mix-culture samples, and then used as the templates for the amplification of 16S rDNA V-3 region. The fingerprinting map of PCR-DGGE, sequencing and phylogenetic analysis all indicated that microbial communities were highly variable in the western West Lake and most microbes existed in very low abundance. The sublake Wuguitan had the highest microbial diversity (represented by 27 DGGE bands). The microbial communities of the physically isolated sub-lakes were similar in the western West Lake while the microbial communities dramatically changed at different sampling sites in the same sublake. The two dominant microorganisms detected by culture-independent PCR-DGGE are closely related to the uncultured bacterium HQ671854.1 and anaerobic bacterium ANA No.40 AB064556.1 while the most abundant microbes were found to be Acinetobacter sp. and Pseudomonas sp. by using culture dependent PCR-DGGE method. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; WASTE-WATER ; BACTERIAL ; BIOFILMS ; MICROORGANISMS ; BIOREACTORS ; DIVERSITY |
| 收录类别 | SCI |
| 资助信息 | National Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005] |
| 语种 | 英语 |
| WOS记录号 | WOS:000305776800002 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16971]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Linlin,Zhou, Qiaohong,He, Feng,et al. INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2012,21(6):1357-1364. |
| APA | Cai, Linlin,Zhou, Qiaohong,He, Feng,Kong, Lingwei,Hu, Shenghua,&Wu, Zhenbin.(2012).INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA.FRESENIUS ENVIRONMENTAL BULLETIN,21(6),1357-1364. |
| MLA | Cai, Linlin,et al."INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA".FRESENIUS ENVIRONMENTAL BULLETIN 21.6(2012):1357-1364. |
入库方式： OAI收割
来源：水生生物研究所
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