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Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae
文献类型：期刊论文
| 作者 | Chen, Qi; Yu, Liqin; Yang, Lihua; Zhou, Bingsheng |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 110页码:141-148 |
| 关键词 | BDE-209 Hypothalamic-Pituitary-Thyroid axis Gene transcription Bioconcentration and metabolism Thyroid endocrine disruption Zebrafish larvae |
| ISSN号 | 0166-445X |
| 通讯作者 | Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Polybrominated diphenyl ethers (PBDEs) have the potential to disturb the thyroid endocrine system, but little is known of such effects or underlying mechanisms of BDE-209 in fish. In the present study, bioconcentration and metabolism of BDE-209 were investigated in zebrafish embryos exposed at concentrations of 0, 0.08, 0.38 and 1.92 mg/L in water until 14 days post-fertilization (dpf). Chemical analysis revealed that BDE-209 was accumulated in zebrafish larvae, while also metabolic products were detected, including octa- and nona-BDEs, with nona-BDEs being predominant. The exposure resulted in alterations of both triiodothyronine (T3) and thyroxine (T4) levels, indicating thyroid endocrine disruption. Gene transcription in the hypothalamic-pituitary-thyroid (HPT) axis was further examined, and the results showed that the genes encoding corticotrophin-releasing hormone (CRH) and thyroid-stimulating hormone (TSH beta) were transcriptionally significantly up-regulated. Genes involved in thyroid development (Pax8 and Nkx2.1) and synthesis (sodium/iodide symporter, NIS, thyroglobulin, TG) were also transcriptionally up-regulated. Up-regulation of mRNA for thyronine deiodinase (Dio1 and Dio2) and thyroid hormone receptors (TR alpha and TR beta) was also observed. However, the genes encoding proteins involved in TH transport (transthyretin, TTR)and metabolism (uridinediphosphate-glucuronosyl-transferase, UGT1ab) were transcriptionally significantly down-regulated. Furthermore, protein synthesis of TG was significantly up-regulated, while that of TTR was significantly reduced. These results suggest that the hypothalamic-pituitary-thyroid axis can be evaluated to determine thyroid endocrine disruption by BDE-209 in developing zebrafish larvae. (C) 2012 Elsevier B.V. All rights reserved. |
| 英文摘要 | Polybrominated diphenyl ethers (PBDEs) have the potential to disturb the thyroid endocrine system, but little is known of such effects or underlying mechanisms of BDE-209 in fish. In the present study, bioconcentration and metabolism of BDE-209 were investigated in zebrafish embryos exposed at concentrations of 0, 0.08, 0.38 and 1.92 mg/L in water until 14 days post-fertilization (dpf). Chemical analysis revealed that BDE-209 was accumulated in zebrafish larvae, while also metabolic products were detected, including octa- and nona-BDEs, with nona-BDEs being predominant. The exposure resulted in alterations of both triiodothyronine (T3) and thyroxine (T4) levels, indicating thyroid endocrine disruption. Gene transcription in the hypothalamic-pituitary-thyroid (HPT) axis was further examined, and the results showed that the genes encoding corticotrophin-releasing hormone (CRH) and thyroid-stimulating hormone (TSH beta) were transcriptionally significantly up-regulated. Genes involved in thyroid development (Pax8 and Nkx2.1) and synthesis (sodium/iodide symporter, NIS, thyroglobulin, TG) were also transcriptionally up-regulated. Up-regulation of mRNA for thyronine deiodinase (Dio1 and Dio2) and thyroid hormone receptors (TR alpha and TR beta) was also observed. However, the genes encoding proteins involved in TH transport (transthyretin, TTR)and metabolism (uridinediphosphate-glucuronosyl-transferase, UGT1ab) were transcriptionally significantly down-regulated. Furthermore, protein synthesis of TG was significantly up-regulated, while that of TTR was significantly reduced. These results suggest that the hypothalamic-pituitary-thyroid axis can be evaluated to determine thyroid endocrine disruption by BDE-209 in developing zebrafish larvae. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | POLYBROMINATED DIPHENYL ETHERS ; PEARL RIVER DELTA ; SPRAGUE-DAWLEY RATS ; PIMEPHALES-PROMELAS ; HORMONE RECEPTORS ; IN-VITRO ; POLYCHLORINATED-BIPHENYLS ; DEVELOPMENTAL EXPOSURE ; GENE-EXPRESSION ; SOUTH CHINA |
| 收录类别 | SCI |
| 资助信息 | National Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01] |
| 语种 | 英语 |
| WOS记录号 | WOS:000302835900017 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16983]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Qi,Yu, Liqin,Yang, Lihua,et al. Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae[J]. AQUATIC TOXICOLOGY,2012,110:141-148. |
| APA | Chen, Qi,Yu, Liqin,Yang, Lihua,&Zhou, Bingsheng.(2012).Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae.AQUATIC TOXICOLOGY,110,141-148. |
| MLA | Chen, Qi,et al."Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae".AQUATIC TOXICOLOGY 110(2012):141-148. |
入库方式： OAI收割
来源：水生生物研究所
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