Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis
文献类型：期刊论文
| 作者 | McLaughlin, Richard William1,2; Chen, MinMin1,3; Zheng, JinSong1,4; Zhao, QingZhong1,4; Wang, Ding1,4 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 39期号:5页码:5669-5676 |
| 关键词 | Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis) 16S rRNA gene Cetacean Bacteria Feces |
| ISSN号 | 0301-4851 |
| 通讯作者 | Zheng, JS (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | The aim of this study was to determine the bacteria present in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis. Fecal samples were collected from 12 Yangtze finless porpoises living in the wild at Poyang Lake, located in Jiangxi Province, China. To determine the bacterial diversity, a 16S rRNA gene clone library using the bacterial PCR primers fD1 and rP2, was prepared. A total of 138 near-full-length sequences were analyzed and 39 operational taxonomic units (OTUs) were identified. Sequences showing a parts per thousand yen97% similarity were grouped together as an OTU. Six different phyla were identified in which 38 OTUs were classified. Most of the OTUs contained sequences belonged to the phylum Firmicutes (51.3%), followed by Tenericutes (17.9%), Proteobacteria (15.4%), Actinobacteria (7.7%), Deinococcus-Thermus (2.6%) and Cyanobacteria (2.6%). A phylum could not be assigned for one clone within one OTU (2.6%). It appears that the Yangtze finless porpoise has a more diverse range of bacteria compared to other aquatic mammals, such as seals. |
| 英文摘要 | The aim of this study was to determine the bacteria present in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis. Fecal samples were collected from 12 Yangtze finless porpoises living in the wild at Poyang Lake, located in Jiangxi Province, China. To determine the bacterial diversity, a 16S rRNA gene clone library using the bacterial PCR primers fD1 and rP2, was prepared. A total of 138 near-full-length sequences were analyzed and 39 operational taxonomic units (OTUs) were identified. Sequences showing a parts per thousand yen97% similarity were grouped together as an OTU. Six different phyla were identified in which 38 OTUs were classified. Most of the OTUs contained sequences belonged to the phylum Firmicutes (51.3%), followed by Tenericutes (17.9%), Proteobacteria (15.4%), Actinobacteria (7.7%), Deinococcus-Thermus (2.6%) and Cyanobacteria (2.6%). A phylum could not be assigned for one clone within one OTU (2.6%). It appears that the Yangtze finless porpoise has a more diverse range of bacteria compared to other aquatic mammals, such as seals. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | CULTURE-INDEPENDENT ANALYSIS ; GAYALS BOS-FRONTALIS ; GASTROINTESTINAL-TRACT ; PHYLOGENETIC ANALYSIS ; MICROBIAL DIVERSITY ; SEQUENCE-ANALYSIS ; INTESTINAL-TRACT ; RUMEN ; CATTLE ; DNA |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[30730018, 31000168]; National Basic Research Program of China[2007CB411600] |
| 语种 | 英语 |
| WOS记录号 | WOS:000302147800071 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16817]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.St Marys Univ Minnesota, Dept Biol, Winona, MN 55987 USA 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | McLaughlin, Richard William,Chen, MinMin,Zheng, JinSong,et al. Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis[J]. MOLECULAR BIOLOGY REPORTS,2012,39(5):5669-5676. |
| APA | McLaughlin, Richard William,Chen, MinMin,Zheng, JinSong,Zhao, QingZhong,&Wang, Ding.(2012).Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis.MOLECULAR BIOLOGY REPORTS,39(5),5669-5676. |
| MLA | McLaughlin, Richard William,et al."Analysis of the bacterial diversity in the fecal material of the endangered Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis".MOLECULAR BIOLOGY REPORTS 39.5(2012):5669-5676. |
入库方式： OAI收割
来源：水生生物研究所
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