Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China)
文献类型：期刊论文
| 作者 | Yan, Qingyun; Yu, Yuhe; Feng, Weisong |
| 刊名 | LIMNOLOGY
 |
| 出版日期 | 2011-12-01 |
| 卷号 | 12期号:3页码:245-251 |
| 关键词 | Nitrogenase iron protein (nifH) gene Biological nitrogen fixation (BNF) Sequences similarity Lake Donghu, Wuhan |
| ISSN号 | 1439-8621 |
| 通讯作者 | Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, 7 Donghu S Rd, Wuhan 430072, Peoples R China. |
| 中文摘要 | Biological nitrogen fixation (BNF) is one of the major nitrogen inputs into the biosphere, and the nitrogenase iron protein (nifH) gene plays important roles in regulating the molecular nitrogen (N-2) fixation process. The nifH gene has also been extensively used to study the diversity and function of nitrogen-fixing microorganisms. In this study, we investigated the diversity of the nifH gene by culture-independent methods to analysis the planktonic nitrogen-fixing organisms in Lake Donghu, Wuhan, the largest urban lake in China. Results indicate that nifH gene sequences cloned from planktonic-community DNA showed high similarity to the uncultured cyanobacterial sequences deposited in the GenBank database. Phylogenetic analysis on the basis of the translated amino acid sequences further showed that most nifH clones were closely related to the reported cyanobacterial nifH gene sequences. Results also indicate that there are similar planktonic nitrogen-fixing organisms in the relatively independent areas of Lake Donghu, even though different regions showed a wide gradient in trophic status. These and other observations led us to believe that studies on nifH gene diversity and expression will increase our ability to understand the ecological function of target nitrogen-fixing groups in aquatic ecosystems. |
| 英文摘要 | Biological nitrogen fixation (BNF) is one of the major nitrogen inputs into the biosphere, and the nitrogenase iron protein (nifH) gene plays important roles in regulating the molecular nitrogen (N-2) fixation process. The nifH gene has also been extensively used to study the diversity and function of nitrogen-fixing microorganisms. In this study, we investigated the diversity of the nifH gene by culture-independent methods to analysis the planktonic nitrogen-fixing organisms in Lake Donghu, Wuhan, the largest urban lake in China. Results indicate that nifH gene sequences cloned from planktonic-community DNA showed high similarity to the uncultured cyanobacterial sequences deposited in the GenBank database. Phylogenetic analysis on the basis of the translated amino acid sequences further showed that most nifH clones were closely related to the reported cyanobacterial nifH gene sequences. Results also indicate that there are similar planktonic nitrogen-fixing organisms in the relatively independent areas of Lake Donghu, even though different regions showed a wide gradient in trophic status. These and other observations led us to believe that studies on nifH gene diversity and expression will increase our ability to understand the ecological function of target nitrogen-fixing groups in aquatic ecosystems. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Limnology |
| 研究领域[WOS] | Marine & Freshwater Biology |
| 关键词[WOS] | NITROGEN-FIXATION ; MARINE ECOSYSTEMS ; FRESH-WATER ; MICROORGANISMS ; LIMITATION ; ESTUARINE ; SEA |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[30770298]; Key Laboratory of Mariculture of Ministry of Education, Ocean University of China[2009004] |
| 语种 | 英语 |
| WOS记录号 | WOS:000300165100005 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16891]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Qingyun,Yu, Yuhe,Feng, Weisong. Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China)[J]. LIMNOLOGY,2011,12(3):245-251. |
| APA | Yan, Qingyun,Yu, Yuhe,&Feng, Weisong.(2011).Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China).LIMNOLOGY,12(3),245-251. |
| MLA | Yan, Qingyun,et al."Diversity of nifH gene amplified from plankton-community DNA in a shallow eutrophic lake (Lake Donghu, Wuhan, China)".LIMNOLOGY 12.3(2011):245-251. |
入库方式： OAI收割
来源：水生生物研究所
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