Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses
文献类型：期刊论文
| 作者 | Chen, Hui1,2; Lu, Yan3; Jiang, Jian-Guo1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012-06-04 |
| 卷号 | 7期号:6页码:e37578 |
| 关键词 | GLYCEROL-3-PHOSPHATE DEHYDROGENASE KLEBSIELLA-PNEUMONIAE BETA-CAROTENE PURIFICATION FERMENTATION TERTIOLECTA REDUCTASE CLONING |
| ISSN号 | 1932-6203 |
| 通讯作者 | Chen, H (reprint author), S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China. |
| 中文摘要 | The glycerol metabolic pathway is a special cycle way; glycerol-3-phosphate dehydrogenase (G3pdh), glycerol-3-phosphate phosphatase (G3pp), dihydroxyacetone reductase (Dhar), and dihydroxyacetone kinase (Dhak) are the key enzymes around the pathway. Glycerol is an important osmolyte for Dunaliella salina to resist osmotic stress. In this study, comparative activities of the four enzymes in D. salina and their activity changes under various salt stresses were investigated, from which glycerol metabolic flow direction in the glycerol metabolic pathway was estimated. Results showed that the salinity changes had different effects on the enzymes activities. NaCl could stimulate the activities of all the four enzymes in various degrees when D. salina was grown under continuous salt stress. When treated by hyperosmotic or hypoosmotic shock, only the activity of G3pdh in D. salina was significantly stimulated. It was speculated that, under osmotic stresses, the emergency response of the cycle pathway in D. salina was driven by G3pdh via its response to the osmotic stress. Subsequently, with the changes of salinity, other three enzymes started to respond to osmotic stress. Dhar played a role of balancing the cycle metabolic pathway by its forward and backward reactions. Through synergy, the four enzymes worked together for the effective flow of the cycle metabolic pathways to maintain the glycerol requirements of cells in order to adapt to osmotic stress environments. |
| 英文摘要 | The glycerol metabolic pathway is a special cycle way; glycerol-3-phosphate dehydrogenase (G3pdh), glycerol-3-phosphate phosphatase (G3pp), dihydroxyacetone reductase (Dhar), and dihydroxyacetone kinase (Dhak) are the key enzymes around the pathway. Glycerol is an important osmolyte for Dunaliella salina to resist osmotic stress. In this study, comparative activities of the four enzymes in D. salina and their activity changes under various salt stresses were investigated, from which glycerol metabolic flow direction in the glycerol metabolic pathway was estimated. Results showed that the salinity changes had different effects on the enzymes activities. NaCl could stimulate the activities of all the four enzymes in various degrees when D. salina was grown under continuous salt stress. When treated by hyperosmotic or hypoosmotic shock, only the activity of G3pdh in D. salina was significantly stimulated. It was speculated that, under osmotic stresses, the emergency response of the cycle pathway in D. salina was driven by G3pdh via its response to the osmotic stress. Subsequently, with the changes of salinity, other three enzymes started to respond to osmotic stress. Dhar played a role of balancing the cycle metabolic pathway by its forward and backward reactions. Through synergy, the four enzymes worked together for the effective flow of the cycle metabolic pathways to maintain the glycerol requirements of cells in order to adapt to osmotic stress environments. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | GLYCEROL-3-PHOSPHATE DEHYDROGENASE ; KLEBSIELLA-PNEUMONIAE ; BETA-CAROTENE ; PURIFICATION ; FERMENTATION ; TERTIOLECTA ; REDUCTASE ; CLONING |
| 收录类别 | SCI |
| 资助信息 | National Natural Foundation of China[30870025] |
| 语种 | 英语 |
| WOS记录号 | WOS:000305341700017 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17039]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 3.S China Univ Technol, Sch Biol Sci & Engn, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hui,Lu, Yan,Jiang, Jian-Guo. Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses[J]. PLOS ONE,2012,7(6):e37578. |
| APA | Chen, Hui,Lu, Yan,&Jiang, Jian-Guo.(2012).Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses.PLOS ONE,7(6),e37578. |
| MLA | Chen, Hui,et al."Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses".PLOS ONE 7.6(2012):e37578. |
入库方式： OAI收割
来源：水生生物研究所
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