Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120
文献类型：期刊论文
| 作者 | Du, Ye; Cai, Yan; Hou, Shengwei; Xu, Xudong |
| 刊名 | JOURNAL OF BACTERIOLOGY
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 194期号:9页码:2297-2306 |
| 关键词 | HETEROCYST PATTERN-FORMATION ESCHERICHIA-COLI SP PCC-7120 GENE-PRODUCT DIFFERENTIATION EXPRESSION CYANOBACTERIA PATA PROTEINS MUTATION |
| ISSN号 | 0021-9193 |
| 通讯作者 | Xu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China. |
| 中文摘要 | HetR is the master regulator of heterocyst differentiation in Anabaena sp. strain PCC 7120 and has been found to specifically bind to an inverted-repeat-containing region upstream of hetP, a heterocyst differentiation gene. However, no such inverted-repeat sequence can be found in promoters of other genes in the genome. hetZ is a gene involved in early heterocyst differentiation. As shown with the gfp reporter gene, transcription from P-hetZ was correlated to the expression level of hetR and inhibition by RGSGR, the pentapeptide derived from the C terminus of PatS. As detected by electrophoretic mobility shift assay, a recombinant HetR showed specific binding to the region upstream of hetZ, and the binding was inhibited by RGSGR. Tests of a series of the upstream fragments delimited the HetR-binding site to a 40-bp region that shows similarity to that upstream of hetP. The introduction of substitutions of bases conserved in the two HetR-binding sites showed that at least 12 bases are required for recognition by HetR. Deletion of a 51-bp region containing the HetR-binding site completely eliminated the transcription activity of P-hetZ. Based on the HetR recognition sequence of hetZ, those upstream of hetR and patA are proposed. |
| 英文摘要 | HetR is the master regulator of heterocyst differentiation in Anabaena sp. strain PCC 7120 and has been found to specifically bind to an inverted-repeat-containing region upstream of hetP, a heterocyst differentiation gene. However, no such inverted-repeat sequence can be found in promoters of other genes in the genome. hetZ is a gene involved in early heterocyst differentiation. As shown with the gfp reporter gene, transcription from P-hetZ was correlated to the expression level of hetR and inhibition by RGSGR, the pentapeptide derived from the C terminus of PatS. As detected by electrophoretic mobility shift assay, a recombinant HetR showed specific binding to the region upstream of hetZ, and the binding was inhibited by RGSGR. Tests of a series of the upstream fragments delimited the HetR-binding site to a 40-bp region that shows similarity to that upstream of hetP. The introduction of substitutions of bases conserved in the two HetR-binding sites showed that at least 12 bases are required for recognition by HetR. Deletion of a 51-bp region containing the HetR-binding site completely eliminated the transcription activity of P-hetZ. Based on the HetR recognition sequence of hetZ, those upstream of hetR and patA are proposed. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | HETEROCYST PATTERN-FORMATION ; ESCHERICHIA-COLI ; SP PCC-7120 ; GENE-PRODUCT ; DIFFERENTIATION ; EXPRESSION ; CYANOBACTERIA ; PATA ; PROTEINS ; MUTATION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001] |
| 语种 | 英语 |
| WOS记录号 | WOS:000302808400018 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17047]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Ye,Cai, Yan,Hou, Shengwei,et al. Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120[J]. JOURNAL OF BACTERIOLOGY,2012,194(9):2297-2306. |
| APA | Du, Ye,Cai, Yan,Hou, Shengwei,&Xu, Xudong.(2012).Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120.JOURNAL OF BACTERIOLOGY,194(9),2297-2306. |
| MLA | Du, Ye,et al."Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120".JOURNAL OF BACTERIOLOGY 194.9(2012):2297-2306. |
入库方式： OAI收割
来源：水生生物研究所
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