Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses
文献类型：期刊论文
| 作者 | Chen, Hui1,2; Chen, Shan-Li1; Jiang, Jian-Guo1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011-12-14 |
| 卷号 | 6期号:12页码:e28613 |
| ISSN号 | 1932-6203 |
| 关键词 | GLYCEROL-3-PHOSPHATE DEHYDROGENASE BETA-CAROTENE ALGAL CELLS CALCIUM ARABIDOPSIS BIOSYNTHESIS EXPRESSION PROTEINS SIGNALS CLONING |
| 通讯作者 | Chen, H (reprint author), S China Univ Technol, Coll Food & Bioengn, Guangzhou, Guangdong, Peoples R China. |
| 中文摘要 | The effect of Ca2+ channel blockers on cytosolic Ca2+ levels and the role of Ca2+ in glycerol metabolism of Dunaliella salina under hypoosmotic or hyperosmotic stress were investigated using the confocal laser scanning microscope (CLSM). Results showed that intracellular Ca2+ concentration increased rapidly when extracellular salinity suddenly decreased or increased, but the increase could be inhibited by pretreatment of Ca2+ channel blockers LaCl3, verapamil or ruthenium red. The changes of glycerol content and G3pdh activity in D. salina to respect to hypoosmotic or hyperosmotic stress were also inhibited in different degrees by pretreatment of Ca2+ channel blockers, indicating that the influx of Ca2+ via Ca2+ channels are required for the transduction of osmotic signal to regulate osmotic responses of D. salina to the changes of salinity. Differences of the three blockers in block effect suggested that they may act on different channels or had different action sites, including influx of Ca2+ from the extracellular space via Ca2+ channels localized in the plasma membrane or from intracellular calcium store via the mitochondrial. Other Ca2+-mediated or non-Ca2+-mediated osmotic signal pathway may exist in Dunaliella in response to hypoosmotic and hyperosmotic stresses. |
| 英文摘要 | The effect of Ca2+ channel blockers on cytosolic Ca2+ levels and the role of Ca2+ in glycerol metabolism of Dunaliella salina under hypoosmotic or hyperosmotic stress were investigated using the confocal laser scanning microscope (CLSM). Results showed that intracellular Ca2+ concentration increased rapidly when extracellular salinity suddenly decreased or increased, but the increase could be inhibited by pretreatment of Ca2+ channel blockers LaCl3, verapamil or ruthenium red. The changes of glycerol content and G3pdh activity in D. salina to respect to hypoosmotic or hyperosmotic stress were also inhibited in different degrees by pretreatment of Ca2+ channel blockers, indicating that the influx of Ca2+ via Ca2+ channels are required for the transduction of osmotic signal to regulate osmotic responses of D. salina to the changes of salinity. Differences of the three blockers in block effect suggested that they may act on different channels or had different action sites, including influx of Ca2+ from the extracellular space via Ca2+ channels localized in the plasma membrane or from intracellular calcium store via the mitochondrial. Other Ca2+-mediated or non-Ca2+-mediated osmotic signal pathway may exist in Dunaliella in response to hypoosmotic and hyperosmotic stresses. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | GLYCEROL-3-PHOSPHATE DEHYDROGENASE ; BETA-CAROTENE ; ALGAL CELLS ; CALCIUM ; ARABIDOPSIS ; BIOSYNTHESIS ; EXPRESSION ; PROTEINS ; SIGNALS ; CLONING |
| 资助信息 | National Natural Foundation of China[30870025] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298369100063 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17063]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.S China Univ Technol, Coll Food & Bioengn, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hui,Chen, Shan-Li,Jiang, Jian-Guo. Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses[J]. PLOS ONE,2011,6(12):e28613. |
| APA | Chen, Hui,Chen, Shan-Li,&Jiang, Jian-Guo.(2011).Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses.PLOS ONE,6(12),e28613. |
| MLA | Chen, Hui,et al."Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses".PLOS ONE 6.12(2011):e28613. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。