Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome
文献类型：期刊论文
| 作者 | Liu, Lusha1,2; Yu, Xiaomu1; Zhu, Chuankun1,2; Zeng, Fanzhen1,2; Tong, Jingou1 |
| 刊名 | CONSERVATION GENETICS RESOURCES
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 4期号:3页码:699-701 |
| 关键词 | Cyprinus carpio L. Single nucleotide polymorphisms (SNPs) Validation Genetic diversity |
| ISSN号 | 1877-7252 |
| 通讯作者 | Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | In this study, we constructed a genomic library to identify a set of new SNPs from the common carp (Cyprinus carpio L.) genome. Putative SNPs were validated by amplification of 41 fragments from the library using unrelated individuals. A total of 211 SNPs, 18 Indels and 6 microsatellites were identified from a total length of 18,244 bp genomic sequences. A/G was the most common substitution, and the ratio of transitions to transversions was 2.3:1. Ten validated SNPs were selected for genetic diversity analysis in three wild populations of common carp, and all loci were polymorphic, with the average observed (H (o)) and expected (H (e)) heterozygosities were 0.2154 and 0.3321, respectively. These new SNPs would be good resources for population genetics, parentage analysis and conservation genetics in common carp. |
| 英文摘要 | In this study, we constructed a genomic library to identify a set of new SNPs from the common carp (Cyprinus carpio L.) genome. Putative SNPs were validated by amplification of 41 fragments from the library using unrelated individuals. A total of 211 SNPs, 18 Indels and 6 microsatellites were identified from a total length of 18,244 bp genomic sequences. A/G was the most common substitution, and the ratio of transitions to transversions was 2.3:1. Ten validated SNPs were selected for genetic diversity analysis in three wild populations of common carp, and all loci were polymorphic, with the average observed (H (o)) and expected (H (e)) heterozygosities were 0.2154 and 0.3321, respectively. These new SNPs would be good resources for population genetics, parentage analysis and conservation genetics in common carp. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biodiversity Conservation ; Genetics & Heredity |
| 研究领域[WOS] | Biodiversity & Conservation ; Genetics & Heredity |
| 收录类别 | SCI |
| 资助信息 | MOA[200903045, 2011-G12]; MOST 973[2010CB126305]; FEBL of China[2011FBZ20] |
| 语种 | 英语 |
| WOS记录号 | WOS:000306586800037 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16905]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lusha,Yu, Xiaomu,Zhu, Chuankun,et al. Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome[J]. CONSERVATION GENETICS RESOURCES,2012,4(3):699-701. |
| APA | Liu, Lusha,Yu, Xiaomu,Zhu, Chuankun,Zeng, Fanzhen,&Tong, Jingou.(2012).Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome.CONSERVATION GENETICS RESOURCES,4(3),699-701. |
| MLA | Liu, Lusha,et al."Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome".CONSERVATION GENETICS RESOURCES 4.3(2012):699-701. |
入库方式： OAI收割
来源：水生生物研究所
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