Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus
文献类型：期刊论文
| 作者 | Shi, Jun; Zhang, Yi-Bing; Liu, Ting-Kai; Sun, Fan; Gui, Jian-Fang |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 33期号:2页码:258-266 |
| 关键词 | Interferon regulator factor 9 Nuclear localization signal Subcellular localization Expression regulation JAK-STAT signaling pathway |
| ISSN号 | 1050-4648 |
| 通讯作者 | Zhang, YB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Luojiashan St, Wuhan 430072, Peoples R China. |
| 中文摘要 | Mammalian interferon (IFN) regulatory factor 9 (IRF-9) has long been recognized as the DNA sequence recognition subunit of IFN-stimulated gene factor 3 (ISGF3) complex, which is critical for type I IFN to induce the expression of IFN-stimulated genes (ISGs) against viral infection. Recent studies have shown that fish IFN exerts antiviral effects by induction of a number of ISGs and also of itself; however, little is known about the role of fish IRF9 in IFN signaling. Here we identify a fish IRF9 orthologue (CaIRF9) from IFN-producing cell line, crucian carp Carassius auratus blastulae embryonic (CAB) cells. Analysis of subcellular distribution of CaIRF9-green fluorescent protein indicates that CaIRF9 is constitutively present in the nucleus, which is driven by two nuclear localization signals (NLS), one locating within DNA-binding domain (DBD) of CaIRF9 and the other immediately behind DBD, although human IRF9 contains only one NLS analogous to the former of CaIRF9. Overexpression of CaIRF9 together with CaSTAT2 not only activates ISRE-containing promoter but also upregulates the expression of fish ISGs. Strikingly, CaIRF9 together with CaSTAT2 also exhibits an ability to activate crucian carp IFN promoter, and blockade of cellular CaIRF9 attenuates IFN itself-induced activation of crucian carp IFN promoter. Taken together, these data suggest that crucian carp IFN induces the expression of ISGs and also of itself possibly by the JAK-STAT signaling pathway that is conserved from fish to mammals. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Mammalian interferon (IFN) regulatory factor 9 (IRF-9) has long been recognized as the DNA sequence recognition subunit of IFN-stimulated gene factor 3 (ISGF3) complex, which is critical for type I IFN to induce the expression of IFN-stimulated genes (ISGs) against viral infection. Recent studies have shown that fish IFN exerts antiviral effects by induction of a number of ISGs and also of itself; however, little is known about the role of fish IRF9 in IFN signaling. Here we identify a fish IRF9 orthologue (CaIRF9) from IFN-producing cell line, crucian carp Carassius auratus blastulae embryonic (CAB) cells. Analysis of subcellular distribution of CaIRF9-green fluorescent protein indicates that CaIRF9 is constitutively present in the nucleus, which is driven by two nuclear localization signals (NLS), one locating within DNA-binding domain (DBD) of CaIRF9 and the other immediately behind DBD, although human IRF9 contains only one NLS analogous to the former of CaIRF9. Overexpression of CaIRF9 together with CaSTAT2 not only activates ISRE-containing promoter but also upregulates the expression of fish ISGs. Strikingly, CaIRF9 together with CaSTAT2 also exhibits an ability to activate crucian carp IFN promoter, and blockade of cellular CaIRF9 attenuates IFN itself-induced activation of crucian carp IFN promoter. Taken together, these data suggest that crucian carp IFN induces the expression of ISGs and also of itself possibly by the JAK-STAT signaling pathway that is conserved from fish to mammals. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | MULTIPLE REGULATORY DOMAINS ; DEPENDENT PROTEIN-KINASE ; PARALICHTHYS-OLIVACEUS ; CELLULAR-LOCALIZATION ; TRANSCRIPTION FACTORS ; ANTIVIRAL RESPONSE ; CELLS RESPOND ; IFN ; EXPRESSION ; VIRUS |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[31172435]; National Key Basic Research Program of China[2010CB126303]; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ17, 2011FBZ24] |
| 语种 | 英语 |
| WOS记录号 | WOS:000306632800012 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16907]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Jun,Zhang, Yi-Bing,Liu, Ting-Kai,et al. Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(2):258-266. |
| APA | Shi, Jun,Zhang, Yi-Bing,Liu, Ting-Kai,Sun, Fan,&Gui, Jian-Fang.(2012).Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus.FISH & SHELLFISH IMMUNOLOGY,33(2),258-266. |
| MLA | Shi, Jun,et al."Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus".FISH & SHELLFISH IMMUNOLOGY 33.2(2012):258-266. |
入库方式： OAI收割
来源：水生生物研究所
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