Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)
文献类型：期刊论文
| 作者 | Lei, Xiao-Ying; Chen, Zhong-Yuan; He, Li-Bo; Pei, Chao; Yuan, Xiu-Ping; Zhang, Qi-Ya |
| 刊名 | FISH PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 38期号:4页码:1175-1182 |
| 关键词 | Red-spotted grouper Epinephelus akaara Skin cell line Chromosome Virus susceptibility Virus protein |
| ISSN号 | 0920-1742 |
| 通讯作者 | Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | A red-spotted grouper Epinephelus akaara skin (RGS) cell line was established and characterized. RGS cells had a normal diploid chromosome number of 2n = 48, the morphology of which was fibroblastic-like in 3 days and epithelial-like over 5 after 16 passages. The cells multiplied well in Dulbecco's modified Eagle's medium supplemented with 10% of fetal bovine serum at 25A degrees C. Susceptibilities of RGS and grass carp ovary (GCO) cells to two viruses were tested, and the results showed that the titer of an iridovirus Rana grylio virus (RGV) in RGS cells was 10(3.5) TCID50 ml(-1), which was much higher than a rhabdovirus spring viremia of carp virus (SVCV) in the cells (10(0.5) TCID50 ml(-1)). The titers of RGV and SVCV in GCO were 10(6.0) TCID50 ml(-1) and 10(8.0) TCID50 ml(-1), respectively, which were higher than those in RGS cells. The data may imply that RGS cells could be selectively resistible to some viruses during infection. RT-PCR analysis of RGV-infected RGS cells showed that RGV could replicate in RGS cells. Further study of virus replications in RGS cells was conducted by electron microscopy and immunofluorescence microscopy has shown that virus particles scattered in the cytoplasm and virus protein appeared in both the cytoplasm and nucleus. The results suggested that RGS cells could be used as a potential in vitro model to study the cutaneous barrier function against virus infection. |
| 英文摘要 | A red-spotted grouper Epinephelus akaara skin (RGS) cell line was established and characterized. RGS cells had a normal diploid chromosome number of 2n = 48, the morphology of which was fibroblastic-like in 3 days and epithelial-like over 5 after 16 passages. The cells multiplied well in Dulbecco's modified Eagle's medium supplemented with 10% of fetal bovine serum at 25A degrees C. Susceptibilities of RGS and grass carp ovary (GCO) cells to two viruses were tested, and the results showed that the titer of an iridovirus Rana grylio virus (RGV) in RGS cells was 10(3.5) TCID50 ml(-1), which was much higher than a rhabdovirus spring viremia of carp virus (SVCV) in the cells (10(0.5) TCID50 ml(-1)). The titers of RGV and SVCV in GCO were 10(6.0) TCID50 ml(-1) and 10(8.0) TCID50 ml(-1), respectively, which were higher than those in RGS cells. The data may imply that RGS cells could be selectively resistible to some viruses during infection. RT-PCR analysis of RGV-infected RGS cells showed that RGV could replicate in RGS cells. Further study of virus replications in RGS cells was conducted by electron microscopy and immunofluorescence microscopy has shown that virus particles scattered in the cytoplasm and virus protein appeared in both the cytoplasm and nucleus. The results suggested that RGS cells could be used as a potential in vitro model to study the cutaneous barrier function against virus infection. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| 关键词[WOS] | RANA-GRYLIO VIRUS ; NF-KAPPA-B ; SPRING VIREMIA ; TELEOST FISH ; CARP VIRUS ; SEA BREAM ; ESTABLISHMENT ; COIOIDES ; IRIDOVIRUS ; ANTIBODIES |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16] |
| 语种 | 英语 |
| WOS记录号 | WOS:000305980200024 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16913]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Xiao-Ying,Chen, Zhong-Yuan,He, Li-Bo,et al. Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2012,38(4):1175-1182. |
| APA | Lei, Xiao-Ying,Chen, Zhong-Yuan,He, Li-Bo,Pei, Chao,Yuan, Xiu-Ping,&Zhang, Qi-Ya.(2012).Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara).FISH PHYSIOLOGY AND BIOCHEMISTRY,38(4),1175-1182. |
| MLA | Lei, Xiao-Ying,et al."Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara)".FISH PHYSIOLOGY AND BIOCHEMISTRY 38.4(2012):1175-1182. |
入库方式： OAI收割
来源：水生生物研究所
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