Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus
文献类型：期刊论文
| 作者 | Yang, Yuhui1; Peng, Kou1; Liu, Xiaoyan1; Zhao, Dongmei1; Duan, Jundan1; Hu, Wei2; Zhao, Haobin1 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2012-06-20 |
| 卷号 | 350页码:1-7 |
| 关键词 | Gobiocypris rarus Myostatin Tempo-spatial expression Real time PCR 17 alpha-ethynyl estradiol (EE2) 17 alpha-methyl testosterone (MT) |
| ISSN号 | 0044-8486 |
| 通讯作者 | Zhao, HB (reprint author), Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China. |
| 中文摘要 | Myostatin (MSTN), belonging to transforming growth factor (TGF) beta superfamily, is a negative regulator of muscular development in mammals and fish. The expression of MSTN can be regulated by hormone and environmental factors. Sex steroids, androgen and estrogen can affect sex differentiation and also can affect muscle development. However, few documents are concerned about the effects of sex steroids on MSTN expression in fish. Here, we report the cloning and expressional analyses of MSTN in rare minnow (Gobiocypris rarus), an emerging model fish in China. The MSTN cDNA of rare minnow that has an open reading frame of 1128 base pairs (bp) encodes a protein of 375 amino acids belonging to fish MSTN1 with typical structures like a prodomain, a mature MSTN domain, a conservative hydrolytic site (RIRR) and 9 conservative cysteine residues in mature MSTN. MSTN was detectable in all the examined tissues by RT-PCR that indicated the ubiquitous expression of MSTN in adult tissues. MSTN was detectable from zygote till hatching showed the maternal deposition of MSTN mRNA from oocytes and continuous expression during embryogenesis. Treatment of rare minnow with 1 mu g/L 17 alpha-methyl testosterone (MT) or 17 alpha-ethynyl estradiol (EE2) significantly impaired the body growth and increased the expression of MSTN mRNA detected by quantitative real time PCR. However, low dosage of MT (10 ng/L) had no significant effects on body growth and MSTN expression. The results suggest that the effects of exogenous sex steroids on growth can be mediated through MSTN. It is also suggested that low concentration of sex steroid (less than 10 ng/L) may have little effect on MSTN expression. (C) 2012 Elsevier B.V. All rights reserved. |
| 英文摘要 | Myostatin (MSTN), belonging to transforming growth factor (TGF) beta superfamily, is a negative regulator of muscular development in mammals and fish. The expression of MSTN can be regulated by hormone and environmental factors. Sex steroids, androgen and estrogen can affect sex differentiation and also can affect muscle development. However, few documents are concerned about the effects of sex steroids on MSTN expression in fish. Here, we report the cloning and expressional analyses of MSTN in rare minnow (Gobiocypris rarus), an emerging model fish in China. The MSTN cDNA of rare minnow that has an open reading frame of 1128 base pairs (bp) encodes a protein of 375 amino acids belonging to fish MSTN1 with typical structures like a prodomain, a mature MSTN domain, a conservative hydrolytic site (RIRR) and 9 conservative cysteine residues in mature MSTN. MSTN was detectable in all the examined tissues by RT-PCR that indicated the ubiquitous expression of MSTN in adult tissues. MSTN was detectable from zygote till hatching showed the maternal deposition of MSTN mRNA from oocytes and continuous expression during embryogenesis. Treatment of rare minnow with 1 mu g/L 17 alpha-methyl testosterone (MT) or 17 alpha-ethynyl estradiol (EE2) significantly impaired the body growth and increased the expression of MSTN mRNA detected by quantitative real time PCR. However, low dosage of MT (10 ng/L) had no significant effects on body growth and MSTN expression. The results suggest that the effects of exogenous sex steroids on growth can be mediated through MSTN. It is also suggested that low concentration of sex steroid (less than 10 ng/L) may have little effect on MSTN expression. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | MESSENGER-RNA EXPRESSION ; ZEBRAFISH DANIO-RERIO ; RAINBOW-TROUT ; 17-ALPHA-ETHINYLESTRADIOL EE2 ; ONCORHYNCHUS-MYKISS ; BODY-COMPOSITION ; GENE-EXPRESSION ; SPARUS-AURATA ; MUSCLE MASS ; IGF-I |
| 收录类别 | SCI |
| 资助信息 | State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18] |
| 语种 | 英语 |
| WOS记录号 | WOS:000305068800001 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16925]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yuhui,Peng, Kou,Liu, Xiaoyan,et al. Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus[J]. AQUACULTURE,2012,350:1-7. |
| APA | Yang, Yuhui.,Peng, Kou.,Liu, Xiaoyan.,Zhao, Dongmei.,Duan, Jundan.,...&Zhao, Haobin.(2012).Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus.AQUACULTURE,350,1-7. |
| MLA | Yang, Yuhui,et al."Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus".AQUACULTURE 350(2012):1-7. |
入库方式： OAI收割
来源：水生生物研究所
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