Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line
文献类型：期刊论文
| 作者 | Zhou, Guang-Zhou1,2; Zhu, Rong1; Gui, Jian-Fang1; Zhang, Qi-Ya1 |
| 刊名 | FISH PATHOLOGY
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 47期号:1页码:30-32 |
| 关键词 | Mandarin fish rhabdovirus nucleoprotein RNA interference SCRV |
| ISSN号 | 0388-788X |
| 通讯作者 | Zhou, GZ (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | We evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection. |
| 英文摘要 | We evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Veterinary Sciences |
| 关键词[WOS] | MANDARIN FISH ; SEQUENCE |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program[2009CB118704]; National 863 High Technology Research Foundation of China[2007AA09Z429, 2006AA09Z445, 20060110A4013]; National Natural Science Foundation of China[30871938, 30800854, 31101931]; Henan University of Technology[2010BS016] |
| 语种 | 英语 |
| WOS记录号 | WOS:000305037500007 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16927]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Guang-Zhou,Zhu, Rong,Gui, Jian-Fang,et al. Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line[J]. FISH PATHOLOGY,2012,47(1):30-32. |
| APA | Zhou, Guang-Zhou,Zhu, Rong,Gui, Jian-Fang,&Zhang, Qi-Ya.(2012).Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line.FISH PATHOLOGY,47(1),30-32. |
| MLA | Zhou, Guang-Zhou,et al."Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line".FISH PATHOLOGY 47.1(2012):30-32. |
入库方式： OAI收割
来源：水生生物研究所
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