The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus
文献类型：期刊论文
| 作者 | Chen, Zhong-Yuan; Lei, Xiao-Ying; Zhang, Qi-Ya |
| 刊名 | VETERINARY MICROBIOLOGY
 |
| 出版日期 | 2012-06-15 |
| 卷号 | 157期号:3-4页码:264-275 |
| 关键词 | Mandarin fish Rhabdovirus DNA vaccination Antiviral defense mechanisms |
| ISSN号 | 0378-1135 |
| 通讯作者 | Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Plasmid DNAs containing Siniperca chuatsi rhabdovirus (SCRV) glycoprotein gene (pcDNA-G) and nucleoprotein gene (pcDNA-N) were constructed, and used to determine the antiviral immune response elicited by DNA vaccination in mandarin fish. In vitro and in vivo expression of the plasmid constructs was confirmed in transfected cells and muscle tissues of vaccinated fish by Western blot, indirect immunofluorescence or RT-PCR analysis. Fish injected with pcDNA-G exhibited protective effect against SCRV challenge with a relative percent survival (RPS) of 77.5%, but no significant protection (RPS of 2.5%) was observed in fish vaccinated with pcDNA-N. Immunohistochemical analysis showed that vaccination with pcDNA-G decreased histological lesions and suppressed the virus replication in fish target organs, e.g. kidney, liver, spleen, gill and heart. Transcriptional analysis further revealed that the expression levels of type I IFN system genes including interferon regulation factor-7 (IRF-7) gene, myxovirus resistance (Mx) gene and virus inhibitory protein (Viperin) gene were strongly up-regulated after injection with pcDNA-G, whereas the level of transcription of immunoglobulin M (IgM) gene did not show a statistically significant change. These results reveal that type I IFN antiviral immune response is rapidly triggered by the plasmid DNA containing rhabdovirus glycoprotein gene in fish, which offers an explanation of molecular mechanisms for DNA vaccination inducing mandarin fish resist to SCRV disease. (C) 2011 Elsevier B.V. All rights reserved. |
| 英文摘要 | Plasmid DNAs containing Siniperca chuatsi rhabdovirus (SCRV) glycoprotein gene (pcDNA-G) and nucleoprotein gene (pcDNA-N) were constructed, and used to determine the antiviral immune response elicited by DNA vaccination in mandarin fish. In vitro and in vivo expression of the plasmid constructs was confirmed in transfected cells and muscle tissues of vaccinated fish by Western blot, indirect immunofluorescence or RT-PCR analysis. Fish injected with pcDNA-G exhibited protective effect against SCRV challenge with a relative percent survival (RPS) of 77.5%, but no significant protection (RPS of 2.5%) was observed in fish vaccinated with pcDNA-N. Immunohistochemical analysis showed that vaccination with pcDNA-G decreased histological lesions and suppressed the virus replication in fish target organs, e.g. kidney, liver, spleen, gill and heart. Transcriptional analysis further revealed that the expression levels of type I IFN system genes including interferon regulation factor-7 (IRF-7) gene, myxovirus resistance (Mx) gene and virus inhibitory protein (Viperin) gene were strongly up-regulated after injection with pcDNA-G, whereas the level of transcription of immunoglobulin M (IgM) gene did not show a statistically significant change. These results reveal that type I IFN antiviral immune response is rapidly triggered by the plasmid DNA containing rhabdovirus glycoprotein gene in fish, which offers an explanation of molecular mechanisms for DNA vaccination inducing mandarin fish resist to SCRV disease. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology ; Veterinary Sciences |
| 研究领域[WOS] | Microbiology ; Veterinary Sciences |
| 关键词[WOS] | HEMATOPOIETIC NECROSIS VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; PARALICHTHYS-OLIVACEUS ; SPRING VIREMIA ; TIME-COURSE ; CARP VIRUS ; VACCINES ; GENE ; EXPRESSION |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program[2009CB118704, 2010CB126303]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ16]; Chinese Academy of Sciences[2010113] |
| 语种 | 英语 |
| WOS记录号 | WOS:000304643800002 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16929]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhong-Yuan,Lei, Xiao-Ying,Zhang, Qi-Ya. The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus[J]. VETERINARY MICROBIOLOGY,2012,157(3-4):264-275. |
| APA | Chen, Zhong-Yuan,Lei, Xiao-Ying,&Zhang, Qi-Ya.(2012).The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus.VETERINARY MICROBIOLOGY,157(3-4),264-275. |
| MLA | Chen, Zhong-Yuan,et al."The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus".VETERINARY MICROBIOLOGY 157.3-4(2012):264-275. |
入库方式： OAI收割
来源：水生生物研究所
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