Germ cell specific expression of Vasa in rare minnow, Gobiocypris rarus
文献类型：期刊论文
| 作者 | Cao, Mengxi1,2; Yang, Yuhui2; Xu, Hongyan3; Duan, Jundan2; Cheng, Nana2; Wang, Jialin2; Hu, Wei1; Zhao, Haobin2 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 162期号:3页码:163-170 |
| 关键词 | Rare minnow Germ cells Primordial germ cells vasa Grvas Testis Ovary Embryonic development |
| ISSN号 | 1095-6433 |
| 通讯作者 | Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China. |
| 中文摘要 | Germ cells are set aside early with somatic cells and take roles for reproduction of species from one generation to the next generation. Vasa, a member of DEAD family is well documented as germ cell marker in the animal kingdom. Rare minnow, Gobiocypris rarus, is an emerging model fish in China to study development and toxicology, etc. A suitable germ cell marker will benefit the studies of the factors that may influence germ cell development. Here, we report the cloning and characterization of G. rarus vasa named Grvas whose protein product has the typical characteristics of Vasa proteins. RT-PCR results showed that Grvas is expressed specifically in the gonads of male and female, it is maternally deposited into the eggs for embryos and is continuously expressed in the embryos from the zygote to larvae and adult. Grvas mRNA and/or protein is restricted to the germ cells of ovary and testis. Temporal expression of Grvas mRNA is similar to that of zebrafish vasa during embryogenesis. Grvas signals are coincident with primordial germ cells. These result; mean that a germ cell marker, Grvas is isolated from rare minnow and its expression is exclusively in germ cells. (C) 2012 Elsevier Inc. All rights reserved. |
| 英文摘要 | Germ cells are set aside early with somatic cells and take roles for reproduction of species from one generation to the next generation. Vasa, a member of DEAD family is well documented as germ cell marker in the animal kingdom. Rare minnow, Gobiocypris rarus, is an emerging model fish in China to study development and toxicology, etc. A suitable germ cell marker will benefit the studies of the factors that may influence germ cell development. Here, we report the cloning and characterization of G. rarus vasa named Grvas whose protein product has the typical characteristics of Vasa proteins. RT-PCR results showed that Grvas is expressed specifically in the gonads of male and female, it is maternally deposited into the eggs for embryos and is continuously expressed in the embryos from the zygote to larvae and adult. Grvas mRNA and/or protein is restricted to the germ cells of ovary and testis. Temporal expression of Grvas mRNA is similar to that of zebrafish vasa during embryogenesis. Grvas signals are coincident with primordial germ cells. These result; mean that a germ cell marker, Grvas is isolated from rare minnow and its expression is exclusively in germ cells. (C) 2012 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Physiology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Physiology ; Zoology |
| 关键词[WOS] | MEDAKA ORYZIAS-LATIPES ; GREEN FLUORESCENT PROTEIN ; TESTICULAR DYSGENESIS SYNDROME ; TRANSGENIC RAINBOW-TROUT ; ZEBRAFISH VASA ; MESSENGER-RNA ; MOLECULAR CHARACTERIZATION ; DIFFERENTIAL EXPRESSION ; GENE PROMOTER ; DROSOPHILA |
| 收录类别 | SCI |
| 资助信息 | National Key Laboratory of Fresh Water Ecology and Biotechnology; National Natural Science Foundation of China[31140053]; Hubei Key Laboratory of Genetic Regulation and Integrative Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000304509800002 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16935]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Huazhong Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China 3.Natl Univ Singapore, Dept Biochem, Singapore 117597, Singapore |
| 推荐引用方式 GB/T 7714 | Cao, Mengxi,Yang, Yuhui,Xu, Hongyan,et al. Germ cell specific expression of Vasa in rare minnow, Gobiocypris rarus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2012,162(3):163-170. |
| APA | Cao, Mengxi.,Yang, Yuhui.,Xu, Hongyan.,Duan, Jundan.,Cheng, Nana.,...&Zhao, Haobin.(2012).Germ cell specific expression of Vasa in rare minnow, Gobiocypris rarus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,162(3),163-170. |
| MLA | Cao, Mengxi,et al."Germ cell specific expression of Vasa in rare minnow, Gobiocypris rarus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 162.3(2012):163-170. |
入库方式： OAI收割
来源：水生生物研究所
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