EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis
文献类型：期刊论文
| 作者 | Cao, Hong1; Leng, Xiaoqian2; Li, Chuangju3; Wei, Qiwei3; Gui, Jianfang2; Cheng, Hanhua1; Zhou, Rongjia1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 39期号:4页码:4647-4653 |
| 关键词 | Endocrinology ESTs Somatolactin Acipenser sinensis Pituitary |
| ISSN号 | 0301-4851 |
| 通讯作者 | Cheng, HH (reprint author), Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China. |
| 中文摘要 | Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon. |
| 英文摘要 | Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | GROWTH-HORMONE ; CDNA CLONING ; COHO SALMON ; ONCORHYNCHUS-KISUTCH ; EXPRESSION PROFILE ; FISH ; PROLACTIN ; SEQUENCE ; CATFISH ; ALPHA |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China; National Key Basic Research project[2010CB126306] |
| 语种 | 英语 |
| WOS记录号 | WOS:000301108500146 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16955]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist China, Yangtze River Fisheries Res Inst, Jinzhou 434000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Hong,Leng, Xiaoqian,Li, Chuangju,et al. EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis[J]. MOLECULAR BIOLOGY REPORTS,2012,39(4):4647-4653. |
| APA | Cao, Hong.,Leng, Xiaoqian.,Li, Chuangju.,Wei, Qiwei.,Gui, Jianfang.,...&Zhou, Rongjia.(2012).EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis.MOLECULAR BIOLOGY REPORTS,39(4),4647-4653. |
| MLA | Cao, Hong,et al."EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis".MOLECULAR BIOLOGY REPORTS 39.4(2012):4647-4653. |
入库方式： OAI收割
来源：水生生物研究所
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