IDENTIFICATION AND CHARACTERIZATION OF A MITOCHONDRIAL MANGANESE SUPEROXIDE DISMUTASE OF SPIROMETRA ERINACEI
文献类型:期刊论文
作者 | Li, Ai-Hua; Na, Byoung-Kuk; Song, Kyoung-Ju; Lim, Sung-Bin; Chong, Chom-Kyu; Park, Yun-Kyu; Kim, Tong-Soo |
刊名 | JOURNAL OF PARASITOLOGY |
出版日期 | 2011-12-01 |
卷号 | 97期号:6页码:1106-1112 |
ISSN号 | 0022-3395 |
关键词 | PARASITE ONCHOCERCA-VOLVULUS SCHISTOSOMA-MANSONI MOLECULAR-CLONING ESCHERICHIA-COLI TRICHINELLA-PSEUDOSPIRALIS FASCIOLA-HEPATICA FILARIAL NEMATODE OXIDATIVE STRESS TAENIA-SOLIUM IRON |
通讯作者 | Kim, TS (reprint author), Inha Univ, Sch Med, Dept Parasitol, Inchon 400712, South Korea. |
中文摘要 | A gene encoding the manganese superoxide dismutase (Mn-SOD) of Spirometra erinacei was identified, and the biochemical properties of the recombinant enzyme were partially characterized. The S. erinacei Mn-SOD gene consisted of 669 bp, which encoded 222 amino acids. A sequence analysis of the gene showed that it had typical molecular structures, including characteristic metal-binding residues and motifs that were conserved in Mn-SODs. An analysis of the N-terminal presequence of S. erinacei Mn-SOD revealed that it had physiochemical characteristics commonly found in mitochondria-targeting sequences and predicted that the enzyme is located in the mitochondria. A biochemical analysis also revealed that the enzyme is a typical Mn-SOD. The enzyme was consistently expressed in both S. erinacei plerocercoid larvae and adult worms. Our results collectively suggested that S. erinacei Mn-SOD is a typical mitochondrial Mn-SOD and may play an important role in parasite physiology, detoxifying excess superoxide radicals generated in the mitochondria. |
英文摘要 | A gene encoding the manganese superoxide dismutase (Mn-SOD) of Spirometra erinacei was identified, and the biochemical properties of the recombinant enzyme were partially characterized. The S. erinacei Mn-SOD gene consisted of 669 bp, which encoded 222 amino acids. A sequence analysis of the gene showed that it had typical molecular structures, including characteristic metal-binding residues and motifs that were conserved in Mn-SODs. An analysis of the N-terminal presequence of S. erinacei Mn-SOD revealed that it had physiochemical characteristics commonly found in mitochondria-targeting sequences and predicted that the enzyme is located in the mitochondria. A biochemical analysis also revealed that the enzyme is a typical Mn-SOD. The enzyme was consistently expressed in both S. erinacei plerocercoid larvae and adult worms. Our results collectively suggested that S. erinacei Mn-SOD is a typical mitochondrial Mn-SOD and may play an important role in parasite physiology, detoxifying excess superoxide radicals generated in the mitochondria. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Parasitology |
研究领域[WOS] | Parasitology |
关键词[WOS] | PARASITE ONCHOCERCA-VOLVULUS ; SCHISTOSOMA-MANSONI ; MOLECULAR-CLONING ; ESCHERICHIA-COLI ; TRICHINELLA-PSEUDOSPIRALIS ; FASCIOLA-HEPATICA ; FILARIAL NEMATODE ; OXIDATIVE STRESS ; TAENIA-SOLIUM ; IRON |
资助信息 | Inha University |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000299022100023 |
公开日期 | 2012-09-25 |
源URL | [http://ir.ihb.ac.cn/handle/342005/16959] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Ai-Hua,Na, Byoung-Kuk,Song, Kyoung-Ju,et al. IDENTIFICATION AND CHARACTERIZATION OF A MITOCHONDRIAL MANGANESE SUPEROXIDE DISMUTASE OF SPIROMETRA ERINACEI[J]. JOURNAL OF PARASITOLOGY,2011,97(6):1106-1112. |
APA | Li, Ai-Hua.,Na, Byoung-Kuk.,Song, Kyoung-Ju.,Lim, Sung-Bin.,Chong, Chom-Kyu.,...&Kim, Tong-Soo.(2011).IDENTIFICATION AND CHARACTERIZATION OF A MITOCHONDRIAL MANGANESE SUPEROXIDE DISMUTASE OF SPIROMETRA ERINACEI.JOURNAL OF PARASITOLOGY,97(6),1106-1112. |
MLA | Li, Ai-Hua,et al."IDENTIFICATION AND CHARACTERIZATION OF A MITOCHONDRIAL MANGANESE SUPEROXIDE DISMUTASE OF SPIROMETRA ERINACEI".JOURNAL OF PARASITOLOGY 97.6(2011):1106-1112. |
入库方式: OAI收割
来源:水生生物研究所
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