中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China

文献类型:期刊论文

作者Liu, J. Y.1,2; Li, A. H.1
刊名JOURNAL OF FISH DISEASES
出版日期2012-05-01
卷号35期号:5页码:335-342
关键词Aeromonas schubertii China infection snakehead
ISSN号0140-7775
通讯作者Li, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要An epizootic in snakehead fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 3545 cm in length. Multiple, ivory-white, firm nodules, 0.51 mm in diameter, were scattered throughout the kidney. Blood clots, 35 mm in diameter, were found in the liver. This is a disease frequently found in cultured snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.31.0 mu m. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these snakehead isolates are identical to A.similar to chubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard KirbyBauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in snakehead fingerlings and zebrafish, Brachydanio rerio (Hamilton).
英文摘要An epizootic in snakehead fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 3545 cm in length. Multiple, ivory-white, firm nodules, 0.51 mm in diameter, were scattered throughout the kidney. Blood clots, 35 mm in diameter, were found in the liver. This is a disease frequently found in cultured snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.31.0 mu m. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these snakehead isolates are identical to A.similar to chubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard KirbyBauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in snakehead fingerlings and zebrafish, Brachydanio rerio (Hamilton).
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]GENUS AEROMONAS ; PHYLOGENETIC ANALYSIS ; NUCLEOTIDE-SEQUENCES ; ALIGNMENT ; STRAINS ; GYRB ; IDENTIFICATION ; HYBRIDIZATION ; NOCARDIOSIS ; PATHOGENS
收录类别SCI
资助信息National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705]
语种英语
WOS记录号WOS:000302615000002
公开日期2012-09-25
源URL[http://ir.ihb.ac.cn/handle/342005/16963]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Liu, J. Y.,Li, A. H.. First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China[J]. JOURNAL OF FISH DISEASES,2012,35(5):335-342.
APA Liu, J. Y.,&Li, A. H..(2012).First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China.JOURNAL OF FISH DISEASES,35(5),335-342.
MLA Liu, J. Y.,et al."First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China".JOURNAL OF FISH DISEASES 35.5(2012):335-342.

入库方式: OAI收割

来源:水生生物研究所

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