First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China
文献类型：期刊论文
| 作者 | Liu, J. Y.1,2; Li, A. H.1 |
| 刊名 | JOURNAL OF FISH DISEASES
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 35期号:5页码:335-342 |
| 关键词 | Aeromonas schubertii China infection snakehead |
| ISSN号 | 0140-7775 |
| 通讯作者 | Li, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | An epizootic in snakehead fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 3545 cm in length. Multiple, ivory-white, firm nodules, 0.51 mm in diameter, were scattered throughout the kidney. Blood clots, 35 mm in diameter, were found in the liver. This is a disease frequently found in cultured snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.31.0 mu m. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these snakehead isolates are identical to A.similar to chubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard KirbyBauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in snakehead fingerlings and zebrafish, Brachydanio rerio (Hamilton). |
| 英文摘要 | An epizootic in snakehead fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 3545 cm in length. Multiple, ivory-white, firm nodules, 0.51 mm in diameter, were scattered throughout the kidney. Blood clots, 35 mm in diameter, were found in the liver. This is a disease frequently found in cultured snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.31.0 mu m. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these snakehead isolates are identical to A.similar to chubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard KirbyBauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in snakehead fingerlings and zebrafish, Brachydanio rerio (Hamilton). |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | GENUS AEROMONAS ; PHYLOGENETIC ANALYSIS ; NUCLEOTIDE-SEQUENCES ; ALIGNMENT ; STRAINS ; GYRB ; IDENTIFICATION ; HYBRIDIZATION ; NOCARDIOSIS ; PATHOGENS |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705] |
| 语种 | 英语 |
| WOS记录号 | WOS:000302615000002 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16963]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, J. Y.,Li, A. H.. First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China[J]. JOURNAL OF FISH DISEASES,2012,35(5):335-342. |
| APA | Liu, J. Y.,&Li, A. H..(2012).First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China.JOURNAL OF FISH DISEASES,35(5),335-342. |
| MLA | Liu, J. Y.,et al."First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China".JOURNAL OF FISH DISEASES 35.5(2012):335-342. |
入库方式： OAI收割
来源：水生生物研究所
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