Poly-3-hydroxybutyrate production from methanol by Methylosinus trichosporium IMV3011 in the non-sterilized fed-batch fermentation
文献类型：期刊论文
| 作者 | Xia CG(夏春谷) ; Song H(宋昊); Xia CG(夏春谷)
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| 刊名 | Afri. J. Microbiol. Res.
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| 出版日期 | 2011 |
| 卷号 | 5页码:5022-5029 |
| 关键词 | Biosynthesis poly-3-hydroxybutyrate methanotrophic bacteria methanol fed-batch fermentation |
| ISSN号 | 1996-0808 |
| 通讯作者 | 夏春谷 |
| 中文摘要 | The methanotrophic bacteria have promising applications in the production of a biopolymer, poly-3-hydroxybutyrate (PHB). The fed-batch fermentation strategies were studied for PHB accumulation by methanol-grown Methylosinus trichosporium IMV3011 under the non-sterilized conditions. The biomass of 2.91 g dry wt/L and PHB accumulation of 47.6% (w/w) were reached in the fed-batch fermentation. In order to increase PHB content, some organic acids were added into the culture as inhibitors of tricarboxylic acid cycle (TCA). It was found that the malic acid should be optimum for PHB accumulation. The biomass and PHB yield would increase to 3.32 g dry wt/L and 58.5% (w/w), respectively by adding 0.2 g/L malic acid. Other key intermediates in the metabolism such as citric acid, succinic acid and acetyl-CoA, also influenced the cell growth and PHB accumulation to a certain extent. The PHB produced in the fermentation process was of steady quality with molecular weights up to 1.7 × 106 Da. |
| 学科主题 | 均相催化 |
| 收录类别 | SCI |
| 资助信息 | the National Natural Science Foundation of China [(20873034) and (21073050)] |
| 语种 | 英语 |
| WOS记录号 | WOS:000298927000010 |
| 公开日期 | 2012-09-20 |
| 源URL | [http://210.77.64.217/handle/362003/325]  |
| 专题 | 兰州化学物理研究所_OSSO国家重点实验室 |
| 通讯作者 | Xia CG(夏春谷); Xia CG(夏春谷) |
| 推荐引用方式 GB/T 7714 | Xia CG,Song H,Xia CG. Poly-3-hydroxybutyrate production from methanol by Methylosinus trichosporium IMV3011 in the non-sterilized fed-batch fermentation[J]. Afri. J. Microbiol. Res.,2011,5:5022-5029. |
| APA | 夏春谷,宋昊,&夏春谷.(2011).Poly-3-hydroxybutyrate production from methanol by Methylosinus trichosporium IMV3011 in the non-sterilized fed-batch fermentation.Afri. J. Microbiol. Res.,5,5022-5029. |
| MLA | 夏春谷,et al."Poly-3-hydroxybutyrate production from methanol by Methylosinus trichosporium IMV3011 in the non-sterilized fed-batch fermentation".Afri. J. Microbiol. Res. 5(2011):5022-5029. |
入库方式： OAI收割
来源：兰州化学物理研究所
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