Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity
文献类型：期刊论文
| 作者 | Xia CG(夏春谷)
|
| 刊名 | Process Biochemistry
 |
| 出版日期 | 2008 |
| 卷号 | 43页码:1179-1185 |
| 关键词 | Hydrophilic magnetic microspheres Suspension polymerization Candida rugosa lipase Immobilization |
| ISSN号 | 1359-5113 |
| 通讯作者 | 李彦峰 |
| 中文摘要 | Magnetic microspheres were prepared by suspension polymerization of glycidyl methacrylate, ethylene glycol dimethacrylate and vinyl acetate in the presence of oleic acid-coated magnetite, and then, alcoholysed to improve their hydrophilicity. Candida rugosa lipase was covalently immobilized on the hydrophilic magnetic microspheres via the active epoxy groups with the activity yield up to 64.2%. The resulting immobilized lipase had better resistance to pH and temperature inactivation in comparison to free lipase, the adaptive pH and temperature ranges of lipase were widened, and it exhibited good thermal stability and reusability. The resistance of immobilized lipase against urea denaturation was also improved, and the immobilized lipase retained 40% of the activity in the presence of 5 M urea whereas free enzyme retained only 8% of activity. Kinetic parameters were also determined for both immobilized and free lipase. The Km value for immobilized (1.03 mg/ml) was higher than that of the free lipase (0.52 mg/ml), whereas the Vmax value was smaller for the immobilized lipase. |
| 学科主题 | 物理化学 |
| 收录类别 | SCI |
| 资助信息 | the State Key Laboratory for Oxo Synthesis and Selective Oxidation Open Foundation (Grant No. OSSO0602) |
| 语种 | 英语 |
| WOS记录号 | WOS:000260809300001 |
| 公开日期 | 2012-09-21 |
| 源URL | [http://210.77.64.217/handle/362003/571]  |
| 专题 | 兰州化学物理研究所_OSSO国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xia CG. Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity[J]. Process Biochemistry,2008,43:1179-1185. |
| APA | 夏春谷.(2008).Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity.Process Biochemistry,43,1179-1185. |
| MLA | 夏春谷."Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity".Process Biochemistry 43(2008):1179-1185. |
入库方式： OAI收割
来源：兰州化学物理研究所
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