Study on immobilization of lipase onto magneticmicrospheres with epoxy groups
文献类型：期刊论文
| 作者 | Xia CG(夏春谷)
|
| 刊名 | Journal of Magnetism and Magnetic Materials
 |
| 出版日期 | 2009 |
| 卷号 | 321页码:252-258 |
| 关键词 | Magnetic microsphere Suspension polymerization Lipase Enzyme immobilization |
| ISSN号 | 0304-8853 |
| 通讯作者 | 李彦峰 |
| 中文摘要 | Magnetic microspheres were synthesized by the suspension polymerization of glycidyl methacrylate (GMA), methacrylic acid (MAA) and divinyl benzene (DVB) in the presence of oleic acid-coated Fe3O4 nanoparticles. Triacylglycerol lipase from porcine pancreas was covalently immobilized on the magnetic microspheres via the active epoxy groups with the activity yield up to 63%( 72.3%) and enzyme loading of 39(70.5)mg/gsupports. The resulting immobilized lipase had higher optimum temperature compared with those of free lipase and exhibited better thermal, broader pH stability and excellent reusability. Furthermore, the catalyzed capability of immobilized lipase was also investigated by catalyzing synthesis of hexyl acetate and the esterification conversion rate reached to 83%( 72.5%) after 12 h in nonaqueous solvent. |
| 学科主题 | 物理化学 |
| 收录类别 | SCI |
| 资助信息 | the State Key Laboratory for Oxo Synthesis and Selective Oxidation Open Foundation (Grant no. OSSO0602) |
| 语种 | 英语 |
| 公开日期 | 2012-09-21 |
| 源URL | [http://210.77.64.217/handle/362003/594]  |
| 专题 | 兰州化学物理研究所_OSSO国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xia CG. Study on immobilization of lipase onto magneticmicrospheres with epoxy groups[J]. Journal of Magnetism and Magnetic Materials,2009,321:252-258. |
| APA | 夏春谷.(2009).Study on immobilization of lipase onto magneticmicrospheres with epoxy groups.Journal of Magnetism and Magnetic Materials,321,252-258. |
| MLA | 夏春谷."Study on immobilization of lipase onto magneticmicrospheres with epoxy groups".Journal of Magnetism and Magnetic Materials 321(2009):252-258. |
入库方式： OAI收割
来源：兰州化学物理研究所
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