The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs
文献类型：期刊论文
| 作者 | Xia CG(夏春谷) ; Xia CG(夏春谷)
|
| 刊名 | Applied Biochemistry and Biotechnology
 |
| 出版日期 | 2009 |
| 卷号 | 157页码:431-441 |
| 关键词 | Methanotroph Methane monooxygenase Poly-β-hydroxybutyrate Reducing equivalent |
| ISSN号 | 0273-2289 |
| 通讯作者 | 夏春谷 ; 辛嘉英 |
| 中文摘要 | Methanotrophs have promising applications in the epoxidation of some alkenes and some chlorinated hydrocarbons and in the production of a biopolymer, poly-β-hydroxybutyrate (poly-3-hydroxybutyrate; PHB). In contrast with methane monooxygenase (MMO) activity and ability of PHB synthesis of four kinds of methanotrophic bacteria Methylosinus trichosporium OB3b, M. trichosporium IMV3011, Methylococcus capsulatus HD6T, Methylomonas sp. GYJ3, and the mixture of the four kinds of strains, M. trichosporium OB3b is the highest of the four in the activity of propene epoxidation (10.72 nmol/min mg dry weight of cell [dwc]), the activity of naphthalene oxidation (22.7 mmol/mg dwc), and ability in synthesis of PHB(11% PHB content in per gram dry weight of cell in 84 h). It could be feasible to improve the MMO activity by mixing four kinds of methanotrophs. The MMO activity dramatically decreased when the cellular PHB accumulated in the second stage. The reason for this may be the dilution of the MMO system in the cells with increasing PHB contents. It has been found that the PHB contents at the level of 1–5% are beneficial to the cells for maintenance of MMO epoxidation activity when enough PHB have been accumulated. Moreover, it was also found that high particulate methane monooxygenase activity may contribute to the synthesis of PHB in the cell, which could be used to improve the yield of PHB in methanotrophs. |
| 学科主题 | 清洁催化与生物转化 |
| 收录类别 | SCI |
| 资助信息 | the Program for New Century Excellent Talents in University (NCET-05-0358);the National Natural Science Foundation of China (20625308);the Chinese Academy of Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000267662600007 |
| 公开日期 | 2012-09-21 |
| 源URL | [http://210.77.64.217/handle/362003/596]  |
| 专题 | 兰州化学物理研究所_OSSO国家重点实验室 |
| 通讯作者 | Xia CG(夏春谷); Xia CG(夏春谷) |
| 推荐引用方式 GB/T 7714 | Xia CG,Xia CG. The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs[J]. Applied Biochemistry and Biotechnology,2009,157:431-441. |
| APA | 夏春谷,&夏春谷.(2009).The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs.Applied Biochemistry and Biotechnology,157,431-441. |
| MLA | 夏春谷,et al."The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs".Applied Biochemistry and Biotechnology 157(2009):431-441. |
入库方式： OAI收割
来源：兰州化学物理研究所
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