Real time observation of the photocleavage of single DNA molecules
文献类型：期刊论文
| 作者 | Li, B（李宾） ; Hu, J（胡钧） ; Wang, Y（汪颖） ; Wu, SY ; Huang, YB（黄一波） ; Li, MQ（李民乾） |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2003 |
| 卷号 | 48期号:7页码:673 |
| ISSN号 | 1001-6538 |
| 英文摘要 | A method for real time observation of photo-cleavage of stretched lambdaDNA at single molecular level by a fluorescent microscope coupled with CCD is developed. DNA molecules stained with YOYO-1 are stretched by the molecular combing technique and fixed on a modified slide. Then the process of photocleavage and relaxation of DNA under radiation of blue light is observed. We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleavage DNA molecules. The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity, cancer research as well as the interaction between DNA and dyes. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000182411500015 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/10143]  |
| 专题 | 上海应用物理研究所_中科院上海原子核所2003年前 |
| 推荐引用方式 GB/T 7714 | Li, B,Hu, J,Wang, Y,et al. Real time observation of the photocleavage of single DNA molecules[J]. CHINESE SCIENCE BULLETIN,2003,48(7):673. |
| APA | Li, B,Hu, J,Wang, Y,Wu, SY,Huang, YB,&Li, MQ.(2003).Real time observation of the photocleavage of single DNA molecules.CHINESE SCIENCE BULLETIN,48(7),673. |
| MLA | Li, B,et al."Real time observation of the photocleavage of single DNA molecules".CHINESE SCIENCE BULLETIN 48.7(2003):673. |
入库方式： OAI收割
来源：上海应用物理研究所
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