Study on the photo-generation of superoxide radicals in Photosystem II with EPR spin trapping techniques
文献类型：期刊论文
| 作者 | Zhang, SP ; Weng, J ; Pan, JX（潘景喜） ; Tu, TC（屠铁成） ; Yao, S（姚思德） ; Xu, CH |
| 刊名 | PHOTOSYNTHESIS RESEARCH
 |
| 出版日期 | 2003 |
| 卷号 | 75期号:1页码:41 |
| ISSN号 | 0166-8595 |
| 英文摘要 | Direct EPR evidence of the photo-generation of superoxide radicals (O-2(-.)) was obtained by using a novel spin trapping probe in spinach Photosystem II (PS II) membrane fragments. The production of O-2(-.) was detected by following the formation of 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) superoxide adducts (DEPMPO-OOH). The inhibition of O-2(-.) formation by 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and the 77 K fluorescence spectrum indicated that O-2(-.) were generated from PS II, not from PS I. The inhibition of O-2(-.) formation by DCMU also suggested that O-2(-.) were generated from the Q(B)binding site, not at a site prior to DCMU blockage. The extrinsic proteins and Mn are very important to eliminate O-2(-.), showing that the oxygen-evolving system is involved in O-2(-.) removal rather than production. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181045300004 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/10201]  |
| 专题 | 上海应用物理研究所_中科院上海原子核所2003年前 |
| 推荐引用方式 GB/T 7714 | Zhang, SP,Weng, J,Pan, JX,et al. Study on the photo-generation of superoxide radicals in Photosystem II with EPR spin trapping techniques[J]. PHOTOSYNTHESIS RESEARCH,2003,75(1):41. |
| APA | Zhang, SP,Weng, J,Pan, JX,Tu, TC,Yao, S,&Xu, CH.(2003).Study on the photo-generation of superoxide radicals in Photosystem II with EPR spin trapping techniques.PHOTOSYNTHESIS RESEARCH,75(1),41. |
| MLA | Zhang, SP,et al."Study on the photo-generation of superoxide radicals in Photosystem II with EPR spin trapping techniques".PHOTOSYNTHESIS RESEARCH 75.1(2003):41. |
入库方式： OAI收割
来源：上海应用物理研究所
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