The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins
文献类型：期刊论文
| 作者 | Zhang, SB ; Huang, J ; Zhao, H ; Zhang, Y（张益） ; Hou, CH ; Cheng, XD ; Jiang, C ; Li, MQ（李民乾） ; Hu, J（胡钧） ; Qian, RL |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2003 |
| 卷号 | 13期号:5页码:351 |
| ISSN号 | 1001-0602 |
| 英文摘要 | Using atomic force microscopy (AFM), the dynamic process of the in vitro nucleosome reconstitution followed by slow dilution from high salt to low salt was visualized. Data showed that the histone octamers were dissociated from DNA at 1MNaCl. When the salt concentration was slowly reduced to 650 mM and 300 mM, the core histones bound to the naked DNA gradually. Once the salt concentration was reduced to 50 mM the classic "beads-on-a-string" structure was clearly visualized. Furthermore, using the technique of the in vitro reconstitution of nucleosome, the mono- and di- nucleosomes were assembled in vitro with both HS2core (- 10681 to - 10970 bp) and NCR2 (-3 72 to - 194 bp) DNA sequences in the 5'flanking sequence of human b-globin gene. Data revealed that HMG 1/2 and HMG14/17 proteins binding to both DNA sequences are changeable following the assembly and disassembly of nucleosomes. We suggest that the changeable binding patterns of HMG 14/17 and HMG1/2 proteins with these regulatory elements may be critical in the process of nucleosome assembly, recruitment of chromatin-modifying activities, and the regulation of human b-globin gene expression. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187509700006 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/10220]  |
| 专题 | 上海应用物理研究所_中科院上海原子核所2003年前 |
| 推荐引用方式 GB/T 7714 | Zhang, SB,Huang, J,Zhao, H,et al. The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins[J]. CELL RESEARCH,2003,13(5):351. |
| APA | Zhang, SB.,Huang, J.,Zhao, H.,Zhang, Y.,Hou, CH.,...&Qian, RL.(2003).The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins.CELL RESEARCH,13(5),351. |
| MLA | Zhang, SB,et al."The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins".CELL RESEARCH 13.5(2003):351. |
入库方式： OAI收割
来源：上海应用物理研究所
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