Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene
文献类型：期刊论文
| 作者 | Tian, Bo; Chen, Yongyan; Li, Dezhu ; Yan, Yuanxin
|
| 刊名 | DNA SEQUENCE
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 17期号:2页码:143-151 |
| 关键词 | Dendrocalamus latiflorus MADS-box gene AGL2-like LEAFY HULL STERILE1 |
| ISSN号 | 1042-5179 |
| 通讯作者 | Li, DZ (reprint author), Chinese Acad Sci, Inst Bot, Lab Biodivers & Biogeog, Kunming 650204, Peoples R China. tianbo@mail.kib.ac.cn ; dlchyy@126.com ; dzl@mail.kib.ac.cn ; Yanyuanxin@mail.kib.ac.cn |
| 英文摘要 | A cDNA named DlMADS8 was isolated from the young spikelets of the sweet bamboo, Dendrocalamus latiflorus by rapid amplification of cDNA end (RACE). DNA sequence analysis showed that DlMADS8 was composed of full ORF and 3'UTR, but without 5'UTR. The cDNA contained 1059 nucleotides and encoded a putative protein of 244 amino acid residues. The gene displayed the structure of a typical plant MADS-box gene, which consisted of a MADS domain, K domain, a short I region, and the C-terminal region. Phylogenetic analysis of plant MADS-box genes based on amino acid sequences revealed that DlMADS8 was grouped into the AGAMOUS-LIKE 2 (AGL2)-like subfamily. It was homologous to the LEAFY HULL STERILE1 (LHS1) genes of grasses. To study the functions of it, DIMADS8 cDNA clone driven by the CaMV 35S promoter was transformed into Arabidopsis thaliana. Transgenic plants of DIMADS8 exhibited the phenotypes of curled leaves and early flowering. After bolting, three novel phenotypes related to inflorescence development were observed in different transgenic plants. No obvious homeotic conversions of floral organs were observed in all of the 35S::DlMADS8 transgenic Arabidopsis plants. These results indicated that DlMADS8 probably plays a role in floral meristem determinacy and is involved in controlling the flowering time of D. latiflorus. |
| 学科主题 | Biotechnology & Applied Microbiology; Genetics & Heredity |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 关键词[WOS] | MADS-BOX GENES ; FLORAL ORGAN IDENTITY ; ECTOPIC EXPRESSION ; FLOWER DEVELOPMENT ; ARABIDOPSIS-THALIANA ; MERISTEM IDENTITY ; DOMAIN PROTEIN ; FAMILY ; EVOLUTION ; TRANSITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240356000009 |
| 公开日期 | 2012-10-12 |
| 源URL | [http://ir.kib.ac.cn:8080/handle/151853/15560]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Lab Biodivers & Biogeog, Kunming 650204, Peoples R China 2.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Bo,Chen, Yongyan,Li, Dezhu,et al. Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene[J]. DNA SEQUENCE,2006,17(2):143-151. |
| APA | Tian, Bo,Chen, Yongyan,Li, Dezhu,&Yan, Yuanxin.(2006).Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene.DNA SEQUENCE,17(2),143-151. |
| MLA | Tian, Bo,et al."Cloning and characterization of a bamboo LEAFY HULL STERILE1 homologous gene".DNA SEQUENCE 17.2(2006):143-151. |
入库方式： OAI收割
来源：昆明植物研究所
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