Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds
文献类型：期刊论文
| 作者 | Li, HM ; Zeng, ZH ; Hu, Z ; Wang, DC |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
 |
| 出版日期 | 1998 |
| 卷号 | 54期号:1页码:137-139 |
| 关键词 | RIBOSOME-INACTIVATING PROTEINS PHYTOLACCA-AMERICANA MACROMOLECULAR CRYSTALLOGRAPHY WEISSENBERG CAMERA GENOMIC CLONE PURIFICATION |
| ISSN号 | 0907-4449 |
| 通讯作者 | Li, HM (reprint author), Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China. |
| 英文摘要 | Pokeweed antiviral protein from seeds (PAP-S) is a ribosome inactivating protein which has lowest toxicity and highest inhibition activity as opposed to other pokeweed antiviral proteins and its three potential glycosylation sites (10, 44, 255) were shown to bind to N-acetylglucosamine. Good quality crystals of PAP-S were grown at high protein concentration (100 mg ml(-1)) and high temperature (306 K). The crystals have space group I222 and cell parameters a = 78.7, b = 85.2 and c = 93.0 Angstrom. An X-ray diffraction data set with resolution up to 1.8 Angstrom was collected. This high-resolution data will help to locate the sugars bound to the protein and provide accurate structural data for understanding structure-function relationships of PAP-S. |
| 学科主题 | Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-10-12 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/15498]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, HM,Zeng, ZH,Hu, Z,et al. Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,1998,54(1):137-139. |
| APA | Li, HM,Zeng, ZH,Hu, Z,&Wang, DC.(1998).Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,54(1),137-139. |
| MLA | Li, HM,et al."Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 54.1(1998):137-139. |
入库方式： OAI收割
来源：昆明植物研究所
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