Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling
文献类型：期刊论文
| 作者 | Zhou, Jiawei
|
| 刊名 | JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2009 |
| 卷号 | 29期号:24页码:7766-7775 |
| ISSN号 | 0270-6474 |
| 关键词 | D-1 DOPAMINE AGONISTS FIBROBLAST-GROWTH-FACTOR PARKINSONS-DISEASE VARYING EFFICACIES RAT-BRAIN SUBSTANTIA-NIGRA SKF 83959 NEURONS SKF83959 CALCIUM |
| 通讯作者 | Zhou, JW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci, 320 Yueyang Rd, Shanghai 200031, Peoples R China,xczhen@mail.shcnc.ac.cn ; jwzhou@ion.ac.cn |
| 英文摘要 | Fibroblast growth factor-2 (FGF-2) is predominantly synthesized and secreted by astrocytes in adult brain. Our previous study showed that activation of classical dopamine receptor D-1 or D-2 elicits FGF-2 biosynthesis and secretion in astrocytes. Here, we report that astrocytic FGF-2 expression is also regulated by phosphatidylinositol (PI)-linked D-1-like receptor. SKF83959, a selective PI-linked D-1-like receptor agonist, upregulates the levels of FGF-2 protein in striatal astrocyte cultures in classical dopamine D-1 and D-2 receptor-independent manner. The conditional medium derived from SKF83959-activated astrocytes promoted the number of TH+ neurons in vitro. Treatment of astrocytes with SKF83959 increased intracellular calcium in two phases. Inhibition of intracellular calcium oscillation by inositol 1,4,5-triphosphate (IP3) inhibitors blocked the SKF83959-induced increase in FGF-2 expression. Moreover, intraperitoneal administration of SKF83959 reversed l-methyl-4-phenyl-l,2,3,6-tetrahydropypridine (MPTP)-induced reduction in FGF-2 expression in both the striatum and ventral midbrain and resulted in marked protection of dopaminergic neurons from MPTP-induced neurotoxicity. These results indicate that IP3/Ca2+/calmodulin-dependent protein kinase is an uncharted intracellular signaling pathway that is crucial for the regulation of FGF-2 synthesis in astrocytes. PI-linked D-1-like receptor plays an important role in the regulation of astrocytic FGF-2 expression and neuroprotection which may provide a potential target for the drug discovery in Parkinson's disease. |
| 学科主题 | Neurosciences & Neurology |
| 资助信息 | Chinese Ministry of Science and Technology[2006AA02Z184, 2006AA02A114, 2007AA02Z163]; Shanghai Metropolitan Fund for Research and Development[07DJ14005]; Natural Science Foundation of China[30525041, 30623003, 30721004]; State Key Program for Basic Research of China[2006CB500704, 2009CB522201] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1657]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_基底神经节的发育与退行性疾病研究组 |
| 推荐引用方式 GB/T 7714 | Zhou, Jiawei. Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling[J]. JOURNAL OF NEUROSCIENCE,2009,29(24):7766-7775. |
| APA | Zhou, Jiawei.(2009).Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling.JOURNAL OF NEUROSCIENCE,29(24),7766-7775. |
| MLA | Zhou, Jiawei."Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling".JOURNAL OF NEUROSCIENCE 29.24(2009):7766-7775. |
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来源：上海神经科学研究所
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