Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures
文献类型：期刊论文
| 作者 | Zhou, Jiawei
|
| 刊名 | JOURNAL OF NEUROCHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 108期号:4页码:952-961 |
| ISSN号 | 0022-3042 |
| 关键词 | apomorphine astrocyte FGF-2 promoter gene regulation MZF-1 GROWTH FACTOR-2 EXPRESSION BLOOD-BRAIN-BARRIER PARKINSONS-DISEASE DOPAMINERGIC-NEURONS STRIATAL ASTROCYTES CARDIAC MYOCYTES PROMOTER REGION BASAL GANGLIA FACTOR GENE FIBROBLAST |
| 通讯作者 | Zhou, JW (reprint author), Chinese Acad Sci, Inst Neurosci, State Key Lab Neurobiol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China,jwzhou@ion.ac.cn |
| 英文摘要 | Apomorphine (APO), a potent D1/D2 dopamine receptor agonist, is used as an anti-parkinsonian drug. It stimulates the synthesis and release of multiple trophic factors in mesencephalic and striatal neurons, preventing the loss of dopaminergic neurons in vitro. Furthermore, APO enhances the biosynthesis and release of FGF-2 by activating dopamine receptors in striatal astrocytes, where cAMP/PKA and PKC/MAPK signalling cascades mediate this process. We investigate the effects of APO on the fibroblast growth factor-2 (FGF-2) promoter and its regulation in astrocytes and identify the transcription factor and cis element underlying these effects. In screening for cis-acting elements over the entire region of the FGF-2 promoter stimulated by APO in the astrocytes, a sequence located in the -785/-745 region was found to serve as the cis element. This element was recognized by the human myeloid zinc finger protein 1 (MZF-1) transcription factor. Introducing human MZF-1 plasmid and human MZF-1-specific siRNA has different effects on the FGF-2 promoter. Furthermore, it increases FGF-2 protein expression in HeLa cells and primary astrocytes, indicating that APO stimulates the FGF-2 promoter via the MZF-1 transcription factor. These data suggest that APO can enhance the biosynthesis and release of FGF-2 through the activation of the MZF-1 transcription factor in striatal astrocytes. |
| 学科主题 | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| 资助信息 | Chinese Academy of Sciences, the Chinese Ministry of Science Technology[2006AA02Z184]; Shanghai Metropolitan Fund for Research and Development[07DJ14005, 06DZ22032]; Natural Science Foundation of China[30525041, 30621130075]; State Key Program for Basic Research of China[2006CB500704] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1681]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_基底神经节的发育与退行性疾病研究组 |
| 推荐引用方式 GB/T 7714 | Zhou, Jiawei. Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures[J]. JOURNAL OF NEUROCHEMISTRY,2009,108(4):952-961. |
| APA | Zhou, Jiawei.(2009).Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures.JOURNAL OF NEUROCHEMISTRY,108(4),952-961. |
| MLA | Zhou, Jiawei."Crucial roles of MFZ-1 in the transcriptional regulation of apomorphine-induced modulation of FGF-2 expression in astrocytic cultures".JOURNAL OF NEUROCHEMISTRY 108.4(2009):952-961. |
入库方式： OAI收割
来源：上海神经科学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。