Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles
文献类型：期刊论文
| 作者 | Zhang, Xu
|
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 21期号:5页码:741-753 |
| 关键词 | G-protein-coupled receptors Wnt receptors Ca(2+) channels large dense-core vesicles dorsal root ganglion spinal cord DELTA-OPIOID-RECEPTOR DORSAL-ROOT GANGLIA DENSE-CORE VESICLES PRESYNAPTIC ACTIVE ZONES NEUROPATHIC PAIN SPINAL-CORD SUBCELLULAR-DISTRIBUTION GRANULE BIOGENESIS SENSORY NEURONS CHROMOGRANIN-A |
| ISSN号 | 1001-0602 |
| 通讯作者 | Zhang, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,xu.zhang@ion.ac.cn |
| 英文摘要 | Stimulus-induced exocytosis of large dense-core vesicles (LDCVs) leads to discharge of neuropeptides and fusion of LDCV membranes with the plasma membrane. However, the contribution of LDCVs to the properties of the neuronal membrane remains largely unclear. The present study found that LDCVs were associated with multiple receptors, channels and signaling molecules, suggesting that neuronal sensitivity is modulated by an LDCV-mediated mechanism. Liquid chromatography-mass spectrometry combined with immunoblotting of subcellular fractions identified 298 proteins in LDCV membranes purified from the dorsal spinal cord, including G-protein-coupled receptors, G-proteins and other signaling molecules, ion channels and trafficking-related proteins. Morphological assays showed that delta-opioid receptor 1 (DOR1), beta 2 adrenergic receptor (AR), G(alpha i2), voltage-gated calcium channel alpha 2 delta 1 subunit and P2X purinoceptor 2 were localized in substance P (SP)-positive LDCVs in small-diameter dorsal root ganglion neurons, whereas beta 1 AR, Wnt receptor frizzled 8 and dishevelled 1 were present in SP-negative LDCVs. Furthermore, DOR1/G(alpha i2)/G(beta 1 gamma 5)/phospholipase C beta 2 complexes were associated with LDCVs. Blockade of the DOR1/G(alpha i2) interaction largely abolished the LDCV localization of G(alpha i2) and impaired stimulation-induced surface expression of G(alpha i2). Thus, LDCVs serve as carriers of receptors, ion channels and preassembled receptor signaling complexes, enabling a rapid, activity-dependent modulation of neuronal sensitivity. |
| 学科主题 | Cell Biology |
| 收录类别 | SCI |
| 资助信息 | NNSFC[30770678, 30621062]; National Basic Research Program of China[2009CB522005, 2007CB914501, 2010CB912000, 2011CBA00400]; CAS[GJHZ06] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1540]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_感觉系统研究组 |
| 推荐引用方式 GB/T 7714 | Zhang, Xu. Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles[J]. CELL RESEARCH,2011,21(5):741-753. |
| APA | Zhang, Xu.(2011).Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles.CELL RESEARCH,21(5),741-753. |
| MLA | Zhang, Xu."Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles".CELL RESEARCH 21.5(2011):741-753. |
入库方式： OAI收割
来源：上海神经科学研究所
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