Peripheral nerve injury induces trans-synaptic modification of channels, receptors and signal pathways in rat dorsal spinal cord
文献类型：期刊论文
| 作者 | Zhang, X
|
| 刊名 | EUROPEAN JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2004 |
| 卷号 | 19期号:4页码:871-883 |
| 关键词 | ion channel nerve injury receptor signal transduction spinal dorsal horn Sprague-Dawley rats DEPENDENT CA2+ CHANNEL LAMINAE-I-III NEUROPATHIC PAIN GENE-EXPRESSION CALCIUM-CHANNEL PROTEIN-KINASE SENSORY NEURONS ROOT GANGLION SCIATIC-NERVE UP-REGULATION |
| ISSN号 | 0953-816X |
| 通讯作者 | Xiao, HS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Lab Sensory Syst, Shanghai 200031, Peoples R China,xiaohs@ion.ac.cn |
| 英文摘要 | Peripheral tissue injury-induced central sensitization may result from the altered biochemical properties of spinal dorsal horn. However, peripheral nerve injury-induced modification of genes in the dorsal horn remains largely unknown. Here we identified strong changes of 14 channels, 25 receptors and 42 signal transduction related molecules in Sprague-Dawley rat dorsal spinal cord 14 days after peripheral axotomy by cDNA microarray. Twenty-nine genes were further confirmed by semiquantitative RT-PCR, Northern blotting, in situ hybridization and immunohistochemistry. These regulated genes included Ca2+ channel alpha1E and alpha2/delta1 subunits, alpha subunits for Na+ channel 1 and 6, Na+ channel beta subunit, AMAP receptor GIuR3 and 4, GABA(A) receptor alpha5 subunit, nicotinic acetylcholine receptor alpha5 and beta2 subunits, PKC alpha, betal and delta isozymes, JNK1-3, ERK2-3, p38 MAPK and BatK and Lyn tyrosine-protein kinases, indicating that several signal transduction pathways were activated in dorsal spinal cord by peripheral nerve injury. These results demonstrate that peripheral nerve injury causes phenotypic changes in spinal dorsal horn. Increases in Ca2+ channel alpha2/delta1 subunit, GABA(A) receptor alpha5 subunit, Na+ channels and nicotinic acetylcholine receptors in both dorsal spinal cord and dorsal root ganglia indicate their potential roles in neuropathic pain control. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1976]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_感觉系统研究组 |
| 推荐引用方式 GB/T 7714 | Zhang, X. Peripheral nerve injury induces trans-synaptic modification of channels, receptors and signal pathways in rat dorsal spinal cord[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2004,19(4):871-883. |
| APA | Zhang, X.(2004).Peripheral nerve injury induces trans-synaptic modification of channels, receptors and signal pathways in rat dorsal spinal cord.EUROPEAN JOURNAL OF NEUROSCIENCE,19(4),871-883. |
| MLA | Zhang, X."Peripheral nerve injury induces trans-synaptic modification of channels, receptors and signal pathways in rat dorsal spinal cord".EUROPEAN JOURNAL OF NEUROSCIENCE 19.4(2004):871-883. |
入库方式： OAI收割
来源：上海神经科学研究所
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