TORC1 Regulates Activity-Dependent CREB-Target Gene Transcription and Dendritic Growth of Developing Cortical Neurons
文献类型：期刊论文
| 作者 | Xiong, Zhi-Qi
|
| 刊名 | JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2009 |
| 卷号 | 29期号:8页码:2334-2343 |
| 关键词 | activity CREB dendritic arborization gene transcription development CAM-KINASE-IV SENSITIVE COINCIDENCE DETECTOR CALCIUM REGULATION COACTIVATOR TORC2 IN-VIVO PHOSPHORYLATION ACTIVATION EXPRESSION MECHANISM SIGNALS |
| ISSN号 | 0270-6474 |
| 通讯作者 | Zhou, Y (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,yzhou@ion.ac.cn ; xiongzhiqi@ion.ac.cn |
| 英文摘要 | CREB-target gene transcription during neuronal excitation is important for many aspects of neuronal development and function, including dendrite morphogenesis. However, the signaling events that regulate cAMP response element-binding protein (CREB)-mediated gene transcription during dendritic development are not well understood. Herein we report that the CREB coactivator TORC1 (transducer of regulated CREB 1) is required for activity-dependent CREB-target gene expression and dendrite growth in developing cortical neurons. Ca(2+) influx via voltage-gated calcium channels induced TORC1 dephosphorylation and translocation into the nucleus in a calcineurin-dependent manner. Nuclear accumulation of TORC1 initiated the expression of CREB-target genes, including salt-inducible kinase 1 (SIK1). In response of persistent depolarization, de novo SIK1 protein in turn promoted TORC1 phosphorylation and consequent depletion of nucleus-localized TORC1. SIK1 induction thus appears to act as a negative feedback signal that prevents persistent CREB/TORC1-dependent transcription in the face of long-lasting neuronal activity. Overexpressing wild type TORC1 promoted basal as well as activity-induced dendritic growth, whereas expressing a dominant-negative form of TORC1 or downregulating TORC1 inhibited activity-dependent dendritic growth in vitro and in vivo. Together, these results suggest that neuronal activity-dependent dendritic growth in developing cortical neurons relies on transient TORC1-mediated CREB-target gene transcription. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China[2006CB806600]; Key State Research Program of China[2006CB943900]; Innovative Research Group of National Natural Science Foundation of China[30721004]; Chinese Academy of Sciences[KSCX2-YW-R-099]; National Natural Science Foundation of China[30800318] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1676]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_疾病神经生物学研究组 |
| 推荐引用方式 GB/T 7714 | Xiong, Zhi-Qi. TORC1 Regulates Activity-Dependent CREB-Target Gene Transcription and Dendritic Growth of Developing Cortical Neurons[J]. JOURNAL OF NEUROSCIENCE,2009,29(8):2334-2343. |
| APA | Xiong, Zhi-Qi.(2009).TORC1 Regulates Activity-Dependent CREB-Target Gene Transcription and Dendritic Growth of Developing Cortical Neurons.JOURNAL OF NEUROSCIENCE,29(8),2334-2343. |
| MLA | Xiong, Zhi-Qi."TORC1 Regulates Activity-Dependent CREB-Target Gene Transcription and Dendritic Growth of Developing Cortical Neurons".JOURNAL OF NEUROSCIENCE 29.8(2009):2334-2343. |
入库方式： OAI收割
来源：上海神经科学研究所
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