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Wnt1-cre-mediated Conditional Loss of Dicer Results in Malformation of the Midbrain and Cerebellum and Failure of Neural Crest and Dopaminergic Differentiation in Mice
文献类型：期刊论文
| 作者 | Cheng, Leping
|
| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 2期号:3页码:152-163 |
| ISSN号 | 1674-2788 |
| 关键词 | Wnt1-cre Dicer (Dicer1) knockout microRNA neural crest midbrain and hindbrain head skeleton sympathetic ganglia neuropeptide Y dopaminergic neuron development CENTRAL-NERVOUS-SYSTEM ENZYME DICER OLIGODENDROCYTE DIFFERENTIATION NEUROTRANSMITTER PHENOTYPE INT-1 PROTOONCOGENE TISSUE ORIGINS CELL LINEAGE MOUSE-BRAIN MORPHOGENESIS NEURONS |
| 通讯作者 | Cheng, LP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,lpcheng@ion.ac.cn |
| 英文摘要 | The involvement of microRNAs (miRNAs) in the development of the neural crest (NC) cells and other neuronal differentiation is still poorly understood. Here, we investigated the global function of miRNAs in embryonic development by examining the Wnt1-cre-mediated Dicer knockout mice. Dicer ablation resulted in malformation of the midbrain and cerebellum and failure of NC and dopaminergic differentiation. First, the Dicer mutant fetuses exhibited dramatic malformation of the tectum and cerebellum and the eyelids were open. Second, the skeletal structures that are derived from the cranial NC were lost or mostly ablated in Dicer mutant mice. Third, deletion of Dicer in the NC cells resulted in the malformation of the dorsal root ganglia, enteric nervous system and sympathetic ganglia. Interestingly, the expression of neuropeptide Y and its potential regulators TrkA, AP-2 alpha and AP-2 beta was largely abolished in sympathetic neurons of Dicer mutant mice. Fourth, in situ hybridization data revealed that the expression of miR-9, miR-124 and miR-218 in the midbrain and rostral hindbrain area was mostly eliminated in the Dicer mutant mice. We then demonstrated that the development of dopaminergic neurons was impaired in Dicer-deleted mice. Our studies therefore suggest that miRNAs contribute to the embryonic development in multiple locations. |
| 学科主题 | Cell Biology |
| 资助信息 | Ministry of Science and Technology of China[2007CB947102, 2006CB943900]; National Natural Science Foundation of China[30540014, 30770697]; Science and Technology Commission of Shanghai Municipality[07pj14098] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1589]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_神经发育及其调控机理研究组 |
| 推荐引用方式 GB/T 7714 | Cheng, Leping. Wnt1-cre-mediated Conditional Loss of Dicer Results in Malformation of the Midbrain and Cerebellum and Failure of Neural Crest and Dopaminergic Differentiation in Mice[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2010,2(3):152-163. |
| APA | Cheng, Leping.(2010).Wnt1-cre-mediated Conditional Loss of Dicer Results in Malformation of the Midbrain and Cerebellum and Failure of Neural Crest and Dopaminergic Differentiation in Mice.JOURNAL OF MOLECULAR CELL BIOLOGY,2(3),152-163. |
| MLA | Cheng, Leping."Wnt1-cre-mediated Conditional Loss of Dicer Results in Malformation of the Midbrain and Cerebellum and Failure of Neural Crest and Dopaminergic Differentiation in Mice".JOURNAL OF MOLECULAR CELL BIOLOGY 2.3(2010):152-163. |
入库方式： OAI收割
来源：上海神经科学研究所
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