PRESYNAPTIC INHIBITION OF GABAERGIC SYNAPTIC TRANSMISSION BY ADENOSINE IN MOUSE HYPOTHALAMIC HYPOCRETIN NEURONS
文献类型：期刊论文
| 作者 | Xia, J. X. ; Xiong, J. X. ; Wang, H. K. ; Duan, S. M. ; Ye, J. N. ; Hu, Z. A. |
| 刊名 | NEUROSCIENCE
 |
| 出版日期 | 2012 |
| 卷号 | 201页码:46-56 |
| 关键词 | sleep wakefulness adenosine inhibitory postsynaptic currents hypocretin RECEPTOR-MEDIATED MODULATION PURKINJE-CELL SYNAPSES NUCLEUS NEURONS LATERAL HYPOTHALAMUS GLUTAMATE RELEASE OREXIN NEURONS PARALLEL FIBER A1 RECEPTOR IN-VITRO RAT |
| ISSN号 | 0306-4522 |
| 通讯作者 | Ye, JN (reprint author), Xinqiao Hosp, Dept Neurol, 183 Xinqiao St, Chongqing 400037, Peoples R China,jningye@tom.com ; zhianhu@yahoo.com.cn |
| 英文摘要 | Hypocretin neurons in the lateral hypothalamus, a new wakefulness-promoting center, have been recently regarded as an important target involved in endogenous adenosine regulating sleep homeostasis. The GABAergic synaptic transmissions are the main inhibitory afferents to hypocretin neurons, which play an important role in the regulation of excitability of these neurons. The inhibitory effect of adenosine, a homeostatic sleep-promoting factor, on the excitatory glutamatergic synaptic transmissions in hypocretin neurons has been well documented, whether adenosine also modulates these inhibitory GABAergic synaptic transmissions in these neurons has not been investigated. In this study, the effect of adenosine on inhibitory postsynaptic currents (IPSCs) in hypocretin neurons was examined by using perforated patch-clamp recordings in the acute hypothalamic slices. The findings demonstrated that adenosine suppressed the amplitude of evoked IPSCs in a dose-dependent manner, which was completely abolished by 8-cyclopentyltheophylline (CPT), a selective antagonist of adenosine A1 receptor but not adenosine A2 receptor antagonist 3,7-dimethyl-1-(2-propynyl) xanthine. A presynaptic origin was suggested as following: adenosine increased paired-pulse ratio as well as reduced GABAergic miniature IPSC frequency without affecting the miniature IPSC amplitude. Further findings demonstrated that when the frequency of electrical stimulation was raised to 10 Hz, but not 1 Hz, a time-dependent depression of evoked IPSC amplitude was detected in hypocretin neurons, which could be partially blocked by CPT. However, under a higher frequency at 100 Hz stimulation, CPT had no action on the depressed GABAergic synaptic transmission induced by such tetanic stimulation in these hypocretin neurons. These results suggest that endogenous adenosine generated under certain stronger activities of syn-46 aptic transmissions exerts an inhibitory effect on GABAergic synaptic transmission in hypocretin neurons by activation of presynaptic adenosine A1 receptors, which may finely regulate the excitability of these neurons as well as eventually modulate the sleep wakefulness. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[30800450] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1510]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Xia, J. X.,Xiong, J. X.,Wang, H. K.,et al. PRESYNAPTIC INHIBITION OF GABAERGIC SYNAPTIC TRANSMISSION BY ADENOSINE IN MOUSE HYPOTHALAMIC HYPOCRETIN NEURONS[J]. NEUROSCIENCE,2012,201:46-56. |
| APA | Xia, J. X.,Xiong, J. X.,Wang, H. K.,Duan, S. M.,Ye, J. N.,&Hu, Z. A..(2012).PRESYNAPTIC INHIBITION OF GABAERGIC SYNAPTIC TRANSMISSION BY ADENOSINE IN MOUSE HYPOTHALAMIC HYPOCRETIN NEURONS.NEUROSCIENCE,201,46-56. |
| MLA | Xia, J. X.,et al."PRESYNAPTIC INHIBITION OF GABAERGIC SYNAPTIC TRANSMISSION BY ADENOSINE IN MOUSE HYPOTHALAMIC HYPOCRETIN NEURONS".NEUROSCIENCE 201(2012):46-56. |
入库方式： OAI收割
来源：上海神经科学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。