RhoA-ROCK-Myosin pathway regulates morphological plasticity of cultured olfactory ensheathing cells
文献类型：期刊论文
| 作者 | Huang, Zhi-hui ; Wang, Ying ; Yuan, Xiao-bing ; He, Cheng |
| 刊名 | EXPERIMENTAL CELL RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 317期号:20页码:2823-2834 |
| 关键词 | Olfactory ensheathing cells RhoA Morphological plasticity Regeneration Myosin SPINAL-CORD IN-VITRO LYSOPHOSPHATIDIC ACID CYTOSKELETAL PROTEINS ASTROCYTE STELLATION CLINICAL-APPLICATION AXONAL REGENERATION NEURITE RETRACTION NGF-RECEPTOR MIGRATION |
| ISSN号 | 0014-4827 |
| 通讯作者 | Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China,yuanxb@ion.ac.cn ; chenghe@smmu.edu.cn |
| 英文摘要 | Olfactory ensheathing cells (OECs) are glial cells in the olfactory system with morphological and functional plasticity. Cultured OECs have the flattened and process-bearing shape. Reversible changes have been found between these two morphological phenotypes. However, the molecular mechanism underlying the regulation of their morphological plasticity remains elusive. Using RhoA FRET biosensor, we found that the active RhoA signal mainly distributed in the lamellipodia and/or filopodia of OECs. Local disruption of these active RhoA distributions led to the morphological change from the flattened into process-bearing shape and promoted process outgrowth. Furthermore, RhoA pathway inhibitors, Toxin-B, C3, Y-27632 or over-expression of DN-RhoA blocked serum-induced morphological change of OECs from the process-bearing into flattened shape, whereas the activation of RhoA pathway by lysophosphatidic acid (LPA) promoted the morphological change from the process-bearing into flattened shape. Finally, ROCK-Myosin-F-actin as a downstream of RhoA pathway was involved in morphological plasticity of OECs. Taken together, these results suggest that RhoA-ROCK-Myosin pathway mediates the morphological plasticity of cultured OECs in response to extracellular cues. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Oncology ; Cell Biology |
| 收录类别 | SCI |
| 资助信息 | Startup Foundation[QTJ09013]; Wenzhou Science & Technology Bureau[H20100065]; Wenzhou Medical School[XNK07006]; Natural Science Foundation of Zhejiang Province[Y2110242, Y2110364]; National Natural Science Foundation[30770657, 81100903]; National Key Basic Research Program[2007CB947100, 2011CB504401]; Ministry of Science and Technology of China[2009ZX09311-001] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1516]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Huang, Zhi-hui,Wang, Ying,Yuan, Xiao-bing,et al. RhoA-ROCK-Myosin pathway regulates morphological plasticity of cultured olfactory ensheathing cells[J]. EXPERIMENTAL CELL RESEARCH,2011,317(20):2823-2834. |
| APA | Huang, Zhi-hui,Wang, Ying,Yuan, Xiao-bing,&He, Cheng.(2011).RhoA-ROCK-Myosin pathway regulates morphological plasticity of cultured olfactory ensheathing cells.EXPERIMENTAL CELL RESEARCH,317(20),2823-2834. |
| MLA | Huang, Zhi-hui,et al."RhoA-ROCK-Myosin pathway regulates morphological plasticity of cultured olfactory ensheathing cells".EXPERIMENTAL CELL RESEARCH 317.20(2011):2823-2834. |
入库方式： OAI收割
来源：上海神经科学研究所
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