Precise Spatiotemporal Control of Optogenetic Activation Using an Acousto-Optic Device
文献类型：期刊论文
| 作者 | Wang, Zuoren
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011 |
| 卷号 | 6期号:12页码:e28468-e28469 |
| 关键词 | CAENORHABDITIS-ELEGANS OPTICAL INTERROGATION 2-PHOTON EXCITATION NEURONAL-ACTIVITY NEURAL CIRCUITS MOUSE-BRAIN CHANNELRHODOPSIN-2 LIGHT MANIPULATION CHANNEL |
| ISSN号 | 1932-6203 |
| 通讯作者 | Wang, KY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai, Peoples R China,xhzhang@ion.ac.cn ; sqzeng@mail.hust.edu.cn ; zuorenwang@ion.ac.cn |
| 英文摘要 | Light activation and inactivation of neurons by optogenetic techniques has emerged as an important tool for studying neural circuit function. To achieve a high resolution, new methods are being developed to selectively manipulate the activity of individual neurons. Here, we report that the combination of an acousto-optic device (AOD) and single-photon laser was used to achieve rapid and precise spatiotemporal control of light stimulation at multiple points in a neural circuit with millisecond time resolution. The performance of this system in activating ChIEF expressed on HEK 293 cells as well as cultured neurons was first evaluated, and the laser stimulation patterns were optimized. Next, the spatiotemporally selective manipulation of multiple neurons was achieved in a precise manner. Finally, we demonstrated the versatility of this high-resolution method in dissecting neural circuits both in the mouse cortical slice and the Drosophila brain in vivo. Taken together, our results show that the combination of AOD-assisted laser stimulation and optogenetic tools provides a flexible solution for manipulating neuronal activity at high efficiency and with high temporal precision. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 收录类别 | SCI |
| 资助信息 | China 973[2011CBA00404, 2011CBA00403]; NSFC[30927001, 30925013] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1518]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_神经环路与动物行为研究组 |
| 推荐引用方式 GB/T 7714 | Wang, Zuoren. Precise Spatiotemporal Control of Optogenetic Activation Using an Acousto-Optic Device[J]. PLOS ONE,2011,6(12):e28468-e28469. |
| APA | Wang, Zuoren.(2011).Precise Spatiotemporal Control of Optogenetic Activation Using an Acousto-Optic Device.PLOS ONE,6(12),e28468-e28469. |
| MLA | Wang, Zuoren."Precise Spatiotemporal Control of Optogenetic Activation Using an Acousto-Optic Device".PLOS ONE 6.12(2011):e28468-e28469. |
入库方式： OAI收割
来源：上海神经科学研究所
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