Go Signaling in Mushroom Bodies Regulates Sleep in Drosophila
文献类型：期刊论文
| 作者 | Guo, Fang ; Yi, Wei ; Zhou, Mingmin ; Guo, Aike |
| 刊名 | SLEEP
 |
| 出版日期 | 2011 |
| 卷号 | 34期号:3页码:273-U173 |
| 关键词 | Drosophila Go Mushroom bodies cAMP RNAi ASYMMETRIC CELL-DIVISION PROTEIN-KINASE-A CAENORHABDITIS-ELEGANS MOLECULAR CHARACTERIZATION SYNAPTIC TRANSMISSION PERTUSSIS TOXIN BINDING PROTEIN NERVOUS-SYSTEM PDF NEURONS RECEPTOR |
| ISSN号 | 0161-8105 |
| 通讯作者 | Guo, AK (reprint author), Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, 15 Datun Rd, Beijing 100101, Peoples R China,akguo@ion.ac.cn |
| 英文摘要 | Study Objectives: Sleep is a fundamental physiological process and its biological mechanisms are poorly understood. In Drosophila melanogaster, heterotrimeric Go protein is abundantly expressed in the brain. However, its post-developmental function has not been extensively explored. Design: Locomotor activity was measured using the Drosophila Activity Monitoring System under a 12: 12 LD cycle. Sleep was defined as periods of 5 min with no recorded activity. Results: Pan-neuronal elevation of Go signaling induced quiescence accompanied by an increased arousal threshold in flies. By screening region-specific GAL4 lines, we mapped the sleep-regulatory function of Go signaling to mushroom bodies (MBs), a central brain region which modulates memory, decision making, and sleep in Drosophila. Up-regulation of Go activity in these neurons consolidated sleep while inhibition of endogenous Go via expression of Go RNAi or pertussis toxin reduced and fragmented sleep, indicating that the Drosophila sleep requirement is affected by levels of Go activity in the MBs. Genetic interaction results showed that Go signaling serves as a neuronal transmission inhibitor in a cAMP-independent pathway. Conclusion: Go signaling is a novel signaling pathway in MBs that regulates sleep in Drosophila. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 资助信息 | National Science Foundation of China[90820008, 30270341, 30630028, 30621004, 30770511]; National Basic Research Program of China "973" projects[G2000077800, 2006CB806600, 2006CB911003, 2011CB809000]; Knowledge Innovation Engineering Department of the Chinese Academy of Sciences[KJCX1-09-03, KSCX2-YWR-247] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1549]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Guo, Fang,Yi, Wei,Zhou, Mingmin,et al. Go Signaling in Mushroom Bodies Regulates Sleep in Drosophila[J]. SLEEP,2011,34(3):273-U173. |
| APA | Guo, Fang,Yi, Wei,Zhou, Mingmin,&Guo, Aike.(2011).Go Signaling in Mushroom Bodies Regulates Sleep in Drosophila.SLEEP,34(3),273-U173. |
| MLA | Guo, Fang,et al."Go Signaling in Mushroom Bodies Regulates Sleep in Drosophila".SLEEP 34.3(2011):273-U173. |
入库方式： OAI收割
来源：上海神经科学研究所
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