Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish
文献类型：期刊论文
| 作者 | Feng, Yan ; Yan, Tingting ; Zheng, Jin ; Ge, Xinjian ; Mu, Yu ; Zhang, Yi ; Wu, Dongmei ; Du, Jiu-lin ; Zhai, Qiwei |
| 刊名 | JOURNAL OF NEUROSCIENCE RESEARCH
 |
| 出版日期 | 2010 |
| 卷号 | 88期号:15页码:3319-3327 |
| 关键词 | Nmnat2 Wallerian degeneration axon degeneration neurodegeneration NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE WALLERIAN DEGENERATION IN-VIVO ADENINE-DINUCLEOTIDE ENZYMATIC-ACTIVITY NAD BIOSYNTHESIS MODEL SLOW GENE REGENERATION |
| ISSN号 | 0360-4012 |
| 通讯作者 | Zhai, QW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Nutr & Metab, 294 Tai Yuan Rd, Shanghai 200031, Peoples R China,qwzhai@sibs.ac.cn |
| 英文摘要 | Axon degeneration is supposed to be a therapeutic target for treating neurodegenerative diseases. Mauthner cells (M-cells) are ideal for studying axons in vivo because of their limited numbers, large size, and long axons. In this study, we labeled M-cells by single-cell electroporation with plasmids expressing DsRed2 or EGFP. Injury-induced axon degeneration in labeled M-cell was imaged under a confocal microscope, and we found that the Mauthner axons started to degenerate about 24 hr after lesion. The Wld(S) protein containing full-length Nmnat1 is well-known for its axon-protective function in many systems. Overexpression of Wlds in M-cells also greatly delayed axon degeneration in live zebrafish. Nmnat2 is the only Nmnat highly expressed in brain. Here we demonstrated that overexpression of Nmnat2 in M-cells significantly delayed axon degeneration in vivo, and disruption of the NAD synthesis activity of Nmnat2 markedly attenuated its axon-protective function. All these data show that injury-induced axon degeneration of M-cell has a mechanism similar to that in mammalians and would be a valuable model for studying axon degeneration in vivo. (C) 2010 Wiley-Liss, Inc. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[30825009, 30970619]; National Basic Research Program of China (973 Program)[2009CB918403, 2006CB943802, 2007CB914501]; Science and Technology Commission of Shanghai Municipality[07DJ14005, 08QH1402600]; Shanghai Institutes for Biological Sciences[2007KIP103]; Chinese Academy of Sciences |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1565]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Feng, Yan,Yan, Tingting,Zheng, Jin,et al. Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2010,88(15):3319-3327. |
| APA | Feng, Yan.,Yan, Tingting.,Zheng, Jin.,Ge, Xinjian.,Mu, Yu.,...&Zhai, Qiwei.(2010).Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish.JOURNAL OF NEUROSCIENCE RESEARCH,88(15),3319-3327. |
| MLA | Feng, Yan,et al."Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish".JOURNAL OF NEUROSCIENCE RESEARCH 88.15(2010):3319-3327. |
入库方式： OAI收割
来源：上海神经科学研究所
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