热门
Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease
文献类型：期刊论文
| 作者 | Wang, E. -S. ; Sun, Y. ; Guo, J. -G. ; Gao, X. ; Hu, J. -W. ; Zhou, L. ; Hu, J. ; Jiang, C. -C. |
| 刊名 | ACTA NEUROLOGICA SCANDINAVICA
 |
| 出版日期 | 2010 |
| 卷号 | 122期号:5页码:350-359 |
| ISSN号 | 0001-6314 |
| 关键词 | Parkinson's disease cerebrospinal fluid biomarker proteomics DIFFERENCE GEL-ELECTROPHORESIS ALZHEIMERS-DISEASE MULTIPLE-SCLEROSIS PROTEOMIC ANALYSIS NEURODEGENERATIVE DISEASES PROTEIN MARKERS 2-D DIGE CSF BRAIN EXPRESSION |
| 通讯作者 | Jiang, CC (reprint author), Fudan Univ, Huashan Hosp, Dept Neurosurg, 12 Wulumuqi Middle Rd, Shanghai 200040, Peoples R China,wesong@sina.com |
| 英文摘要 | Objective - The application of biomarkers may potentially improve the efficiency of the diagnosis for Parkinson's disease (PD). However, no reliable biomarker has been identified to date. This study is aimed to identify proteins that might serve as potential biomarkers for PD diagnosis or pathogenesis. Materials and methods - Two-dimensional difference gel electrophoresis (2D DIGE) technique, in combination with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), was used to determine the differentially expressed cerebrospinal fluid (CSF) proteins in PD patients (n = 3) compared with normal controls (n = 3). Selected proteins were further confirmed by Western blotting analysis in the CSF of PD patients (n = 8), Alzheimer's disease (AD) patients (n = 6) and normal control subjects (n = 7). Results - Eight proteins were identified after MS and protein database interrogation. In the CSF of PD patients, the expression levels of one isoform of apolipoprotein A-I (apoA-I), tetranectin, myosin phosphatase target subunit 1 (MYPT1), and two unknown proteins were down-regulated, whereas the expression levels of another apoA-I isoform, proapolipoprotein, and lipoprotein were up-regulated. Western blotting indicates that the expression of tetranectin was reduced in the CSF from PD patients and elevated in AD, while the expression of apoA-I was changed only in the CSF from PD patients. Conclusion - Our preliminary results suggest that tetranectin and apoA-I may serve as potential biomarkers for PD, though further validation is needed. |
| 学科主题 | Neurosciences & Neurology |
| 资助信息 | Shanghai Metropolitan Fund for Research and Development[04dz14005]; Shanghai Pujiang Program |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1567]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Wang, E. -S.,Sun, Y.,Guo, J. -G.,et al. Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease[J]. ACTA NEUROLOGICA SCANDINAVICA,2010,122(5):350-359. |
| APA | Wang, E. -S..,Sun, Y..,Guo, J. -G..,Gao, X..,Hu, J. -W..,...&Jiang, C. -C..(2010).Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease.ACTA NEUROLOGICA SCANDINAVICA,122(5),350-359. |
| MLA | Wang, E. -S.,et al."Tetranectin and apolipoprotein A-I in cerebrospinal fluid as potential biomarkers for Parkinson's disease".ACTA NEUROLOGICA SCANDINAVICA 122.5(2010):350-359. |
入库方式： OAI收割
来源：上海神经科学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。