Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis
文献类型：期刊论文
| 作者 | Zhao, Shengli ; Zhou, Yang ; Gross, Jimmy ; Miao, Pei ; Qiu, Li ; Wang, Dongqing ; Chen, Qian ; Feng, Guoping |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2010 |
| 卷号 | 5期号:9页码:e12506-e12507 |
| 关键词 | NEURAL STEM-CELLS CENTRAL-NERVOUS-SYSTEM HIPPOCAMPAL NEUROGENESIS SYNAPTIC PLASTICITY GLUTAMIC-ACID FUNCTIONAL IMPLICATIONS GABAERGIC PHENOTYPE SUBVENTRICULAR ZONE GYRUS NEURONS |
| ISSN号 | 1932-6203 |
| 通讯作者 | Zhao, SL (reprint author), Duke Univ, Med Ctr, Dept Neurobiol, Durham, NC 27710 USA,feng@neuro.duke.edu |
| 英文摘要 | Neurogenesis in the adult hippocampus is an important form of structural plasticity in the brain. Here we report a line of BAC transgenic mice (GAD67-GFP mice) that selectively and transitorily express GFP in newborn dentate granule cells of the adult hippocampus. These GFP+ cells show a high degree of colocalization with BrdU-labeled nuclei one week after BrdU injection and express the newborn neuron marker doublecortin and PSA-NCAM. Compared to mature dentate granule cells, these newborn neurons show immature morphological features: dendritic beading, fewer dendritic branches and spines. These GFP+ newborn neurons also show immature electrophysiological properties: higher input resistance, more depolarized resting membrane potentials, small and non-typical action potentials. The bright labeling of newborn neurons with GFP makes it possible to visualize the details of dendrites, which reach the outer edge of the molecular layer, and their axon (mossy fiber) terminals, which project to the CA3 region where they form synaptic boutons. GFP expression covers the whole developmental stage of newborn neurons, beginning within the first week of cell division and disappearing as newborn neurons mature, about 4 weeks postmitotic. Thus, the GAD67-GFP transgenic mice provide a useful genetic tool for studying the development and regulation of newborn dentate granule cells. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 收录类别 | SCI |
| 资助信息 | Arnold and Mabel Beckman Foundation |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1576]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Zhao, Shengli,Zhou, Yang,Gross, Jimmy,et al. Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis[J]. PLOS ONE,2010,5(9):e12506-e12507. |
| APA | Zhao, Shengli.,Zhou, Yang.,Gross, Jimmy.,Miao, Pei.,Qiu, Li.,...&Feng, Guoping.(2010).Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis.PLOS ONE,5(9),e12506-e12507. |
| MLA | Zhao, Shengli,et al."Fluorescent Labeling of Newborn Dentate Granule Cells in GAD67-GFP Transgenic Mice: A Genetic Tool for the Study of Adult Neurogenesis".PLOS ONE 5.9(2010):e12506-e12507. |
入库方式： OAI收割
来源：上海神经科学研究所
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