Nestin is essential for mitogen-stimulated proliferation of neural progenitor cells
文献类型：期刊论文
| 作者 | Xue, Xiao-jing ; Yuan, Xiao-bing |
| 刊名 | MOLECULAR AND CELLULAR NEUROSCIENCE
 |
| 出版日期 | 2010 |
| 卷号 | 45期号:1页码:26-36 |
| 关键词 | Nestin Neural progenitor cells Proliferation PI3K siRNA INTERMEDIATE-FILAMENT PROTEIN EPIDERMAL-GROWTH-FACTOR CENTRAL-NERVOUS-SYSTEM STEM-CELLS SUBVENTRICULAR ZONE NEUROEPITHELIAL CELLS RADIAL MIGRATION CEREBRAL-CORTEX EXPRESSION NEUROGENESIS |
| ISSN号 | 1044-7431 |
| 通讯作者 | Yuan, XB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China,yuanxb@ion.ac.cn |
| 英文摘要 | The intermediate filament (IF) protein nestin is a widely accepted molecular marker for neural progenitor cells (NPCs), but its function during neurogenesis remains largely unknown. We found that in embryonic cortical NPCs down-regulation of the expression of nestin, but not its co-polymer IF protein vimentin, resulted in a G1 cell-cycle arrest and a severe reduction in the generation of neurons. Furthermore, down-regulating nestin expression in cultured cortical NPCs markedly suppressed their colony-formation ability and blocked the elevation of the cyclin D1/E protein level in response to the treatment with bFGF. Interestingly, nestin down-regulation caused a marked suppression in the activation of the phosphoinositide 3-kinase (PI3K) pathway but not the mitogen-activated protein kinase (MAPK) pathway in these NPCs. Moreover, defects in the proliferation of cortical NPCs caused by nestin down-regulation could be prevented by up-regulating PI3K activity. Thus, nestin is essential for the proliferation of NPCs by promoting the activation of PI3K in response to mitogenic growth factors. (C) 2010 Elsevier Inc. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 资助信息 | 973 projects[2006CB806600, 2006CB943903]; National Natural Science Foundation of China[30625023, 30721004]; Chinese Academy of Sciences[KSCX2-YW-R-103] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1578]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Xue, Xiao-jing,Yuan, Xiao-bing. Nestin is essential for mitogen-stimulated proliferation of neural progenitor cells[J]. MOLECULAR AND CELLULAR NEUROSCIENCE,2010,45(1):26-36. |
| APA | Xue, Xiao-jing,&Yuan, Xiao-bing.(2010).Nestin is essential for mitogen-stimulated proliferation of neural progenitor cells.MOLECULAR AND CELLULAR NEUROSCIENCE,45(1),26-36. |
| MLA | Xue, Xiao-jing,et al."Nestin is essential for mitogen-stimulated proliferation of neural progenitor cells".MOLECULAR AND CELLULAR NEUROSCIENCE 45.1(2010):26-36. |
入库方式： OAI收割
来源：上海神经科学研究所
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