Leading Tip Drives Soma Translocation via Forward F-Actin Flow during Neuronal Migration
文献类型：期刊论文
| 作者 | He, Min ; Zhang, Zheng-hong ; Guan, Chen-bing ; Xia, Di ; Yuan, Xiao-bing |
| 刊名 | JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2010 |
| 卷号 | 30期号:32页码:10885-10898 |
| 关键词 | CEREBELLAR GRANULE CELLS MYOSIN-II GROWTH CONE CORTICAL FLOW RHO-KINASE SIGNALING PATHWAY ANIMAL-CELLS LIGHT-CHAIN DOUBLECORTIN CENTROSOME |
| ISSN号 | 0270-6474 |
| 通讯作者 | Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China,yuanxb@ion.ac.cn |
| 英文摘要 | Neuronal migration involves coordinated extension of the leading process and translocation of the soma, but the relative contribution of different subcellular regions, including the leading process and cell rear, in driving soma translocation remains unclear. By local manipulation of cytoskeletal components in restricted regions of cultured neurons, we examined the molecular machinery underlying the generation of traction force for soma translocation during neuronal migration. In actively migrating cerebellar granule cells in culture, a growth cone (GC)-like structure at the leading tip exhibits high dynamics, and severing the tip or disrupting its dynamics suppressed soma translocation within minutes. Soma translocation was also suppressed by local disruption of F-actin along the leading process but not at the soma, whereas disrupting microtubules along the leading process or at the soma accelerated soma translocation. Fluorescent speckle microscopy using GFP-alpha-actinin showed that a forward F-actin flow along the leading process correlated with and was required for soma translocation, and such F-actin flow depended on myosin II activity. In migrating neurons, myosin II activity was high at the leading tip but low at the soma, and increasing or decreasing this front-to-rear difference accelerated or impeded soma advance. Thus, the tip of the leading process actively pulls the soma forward during neuronal migration through a myosin II-dependent forward F-actin flow along the leading process. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 资助信息 | 973 projects[2006CB806600, 2006CB943903]; National Natural Science Foundation of China[30625023, 30721004]; Chinese Academy of Sciences[KSCX2-YW-R-103] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1581]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | He, Min,Zhang, Zheng-hong,Guan, Chen-bing,et al. Leading Tip Drives Soma Translocation via Forward F-Actin Flow during Neuronal Migration[J]. JOURNAL OF NEUROSCIENCE,2010,30(32):10885-10898. |
| APA | He, Min,Zhang, Zheng-hong,Guan, Chen-bing,Xia, Di,&Yuan, Xiao-bing.(2010).Leading Tip Drives Soma Translocation via Forward F-Actin Flow during Neuronal Migration.JOURNAL OF NEUROSCIENCE,30(32),10885-10898. |
| MLA | He, Min,et al."Leading Tip Drives Soma Translocation via Forward F-Actin Flow during Neuronal Migration".JOURNAL OF NEUROSCIENCE 30.32(2010):10885-10898. |
入库方式： OAI收割
来源：上海神经科学研究所
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