TRPC1 Is Essential for In Vivo Angiogenesis in Zebrafish
文献类型：期刊论文
| 作者 | Yu, Peng-chun ; Gu, Shan-ye ; Bu, Ji-wen ; Du, Jiu-lin |
| 刊名 | CIRCULATION RESEARCH
 |
| 出版日期 | 2010 |
| 卷号 | 106期号:7页码:1221-1232 |
| 关键词 | TRPC1 VEGF ISV angiogenesis zebrafish MICROVASCULAR ENDOTHELIAL-CELLS EMBRYONIC VASCULAR DEVELOPMENT NERVE GROWTH CONES INTRACELLULAR CALCIUM NETWORK FORMATION CA2+ ENTRY VEGF CHANNELS GUIDANCE MECHANISMS |
| ISSN号 | 0009-7330 |
| 通讯作者 | Du, JL (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China,forestdu@ion.ac.cn |
| 英文摘要 | Rationale: Wiring vascular and neural networks are known to share common molecular signaling pathways. Activation of transient receptor potential type C channels (TRPCs) has recently been shown to underlie chemotropic guidance of neural axons. It is thus of interest to examine whether TRPCs are also involved in vascular development. Objective: To determine the role of TRPC1 in angiogenesis in vivo during zebrafish development. Methods and Results: Knockdown of zebrafish trpc1 by antisense morpholino oligonucleotides severely disrupted angiogenic sprouting of intersegmental vessels (ISVs) in zebrafish larvae. This angiogenic defect was prevented by overexpression of a morpholino oligonucleotide-resistant form of zebrafish trpc1 mRNA. Cell transplantation analysis showed that this requirement of Trpc1 for ISV growth was endothelial cell-autonomous. In vivo time-lapse imaging further revealed that the angiogenic defect was attributable to impairment of filopodia extension, migration, and proliferation of ISV tip cells. Furthermore, Trpc1 acted synergistically with vascular endothelial growth factor A (Vegf-a) in controlling ISV growth, and appeared to be downstream to Vegf-a in controlling angiogenesis, as evidence by the findings that Trpc1 was required for Vegf-a-induced ectopic angiogenesis of subintestinal veins and phosphorylation of extracellular signal-regulated kinase. Conclusions: These results provide the first in vivo evidence that TRPC1 is essential for angiogenesis, reminiscent of the role of TRPCs in axon guidance. It implicates that TRPC1 may represent a potential target for treating pathological angiogenesis. (Circ Res. 2010;106:1221-1232.) |
| 学科主题 | Cardiovascular System & Cardiology ; Hematology |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China[2006CB806605]; Key State Research Program of China[2006CB943802]; Shanghai government[06dj14010, 07pj14107] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1599]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Yu, Peng-chun,Gu, Shan-ye,Bu, Ji-wen,et al. TRPC1 Is Essential for In Vivo Angiogenesis in Zebrafish[J]. CIRCULATION RESEARCH,2010,106(7):1221-1232. |
| APA | Yu, Peng-chun,Gu, Shan-ye,Bu, Ji-wen,&Du, Jiu-lin.(2010).TRPC1 Is Essential for In Vivo Angiogenesis in Zebrafish.CIRCULATION RESEARCH,106(7),1221-1232. |
| MLA | Yu, Peng-chun,et al."TRPC1 Is Essential for In Vivo Angiogenesis in Zebrafish".CIRCULATION RESEARCH 106.7(2010):1221-1232. |
入库方式： OAI收割
来源：上海神经科学研究所
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