Critical role of TRPC6 channels in G2 phase transition and the development of human oesophageal cancer
文献类型：期刊论文
| 作者 | Shi, Y. ; Ding, X. ; He, Z-H ; Zhou, K-C ; Wang, Q. ; Wang, Y-Z |
| 刊名 | GUT
 |
| 出版日期 | 2009 |
| 卷号 | 58期号:11页码:1443-1450 |
| 关键词 | CELL-CYCLE SMOOTH-MUSCLE DEPENDENT PATHWAYS CALCIUM-CHANNELS XENOPUS OOCYTES CATION CHANNEL PROLIFERATION PROGRESSION CA2+ ACTIVATION |
| ISSN号 | 0017-5749 |
| 通讯作者 | Wang, YZ (reprint author), Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, Lab Neural Signal Transduct, Shanghai, Peoples R China,doctorwangqun@yahoo.com.cn ; yzwang@ion.ac.cn |
| 英文摘要 | Background: Oesophageal squamous cell carcinoma (OSCC) is one of the leading causes of cancer-related death worldwide. However, the mechanism by which the OSCC develops remains largely unknown. Ion channels are important for cancer development. Whether the transient receptor potential canonical (TRPC), known as the nonselective cation channels, plays a role in OSCC development is unknown. Methods: The expression of TRPC6, a member of TRPC subfamily, was examined in samples from patients with OSCC by immunostaining and in situ hybridisation. The effects of TRPC6 channels on OSCC cell cycle progression, cell growth and in vivo tumour formation were investigated. The functional TRPC6 channels were found in OSCC cells by electrophysiology and Ca(2+) imaging analysis. Results: The expression of TRPC6 at protein and mRNA levels was markedly increased in human OSCC specimens than that in normal human oesophageal tissues. Blockade of TRPC6 channels in human OSCC cells inhibited elevation of intracellular Ca(2+) concentration ([Ca(2+)](i)) and activation of Cdc2 kinase. Meanwhile, the OSCC cell cycle was arrested at G2 phase and the cell growth was suppressed. Furthermore, inhibition of TRPC6 channels suppressed in nude mice the tumour formation generated by injection of the OSCC cells. Conclusion: TRPC6 channels play a critical role in the development of OSCC. The [Ca(2+)](i) elevation regulated by TRPC6 channels is essential for G2 phase progression and OSCC development. These channels might be a novel target for therapeutic intervention of OSCC. |
| 学科主题 | Gastroenterology & Hepatology |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[2006CB806600, 30711120566, U0632006]; Shanghai Leading Academic Discipline Project |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1631]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Shi, Y.,Ding, X.,He, Z-H,et al. Critical role of TRPC6 channels in G2 phase transition and the development of human oesophageal cancer[J]. GUT,2009,58(11):1443-1450. |
| APA | Shi, Y.,Ding, X.,He, Z-H,Zhou, K-C,Wang, Q.,&Wang, Y-Z.(2009).Critical role of TRPC6 channels in G2 phase transition and the development of human oesophageal cancer.GUT,58(11),1443-1450. |
| MLA | Shi, Y.,et al."Critical role of TRPC6 channels in G2 phase transition and the development of human oesophageal cancer".GUT 58.11(2009):1443-1450. |
入库方式： OAI收割
来源：上海神经科学研究所
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