Erythropoietin prevents zinc accumulation and neuronal death after traumatic brain injury in rat hippocampus: In vitro and in vivo studies
文献类型：期刊论文
| 作者 | Zhu, Lin ; Wang, Han-dong ; Yu, Xin-guang ; Jin, Wei ; Qiao, Liang ; Lu, Ting-jia ; Hu, Ze-lan ; Zhou, Jing |
| 刊名 | BRAIN RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 1289页码:96-105 |
| ISSN号 | 0006-8993 |
| 关键词 | Hippocampus Erythropoietin Rat Traumatic brain injury Zinc CEREBRAL-ISCHEMIA CORTICAL-NEURONS ZN2+ ENTRY APOPTOSIS CELLS NEUROPROTECTION EXPRESSION TRANSLOCATION ACTIVATION PROTECTS |
| 通讯作者 | Wang, HD (reprint author), Jinling Hosp, Dept Neurosurg, Nanjing, Jiangsu Prov, Peoples R China,wanghandong61@126.com |
| 英文摘要 | Erythropoietin (Epo) has been gaining great interest for its potential neuroprotective effect in various neurological insults. However, the molecular mechanism underlying how Epo exerts the function is not clear. Recent studies have indicated that Zn(2+) may have a key role in selective cell death in excitotoxicity after injury, In the present study, we studied the effect of recombinant human Epo (rhEpo) in zinc-induced neurotoxicity both in vitro and in vivo. Exposure of cultured hippocampal neurons to 200 mu M ZnCl(2) for 20 min resulted in remarkable neuronal injury, revealed by assessing neuronal morphology. By measuring mitochondrial function using MTT assay, we found that application of rhEpo (0.1 U/ml) 24 h before zinc exposure resulted in a significant increase of neuronal survival (0.6007 +/- 0.2280 Epo group vs 0.2333 +/- 0.1249 in control group; n=4, p<0.01). Furthermore, we demonstrated that administration of rhEpo (5,000 IU/kg, intraperitoneal) 30 min after traumatic brain injury (TBI) in rats dramatically protected neuronal death indicated by ZP4 staining, a new zinc-specific fluorescent sensor which has been widely used to indicate neuronal damage after excitotoxic injury (n=5/group, p<0.05). Neuronal damage was also assessed by Fluoro-Jade B (FJB) staining, a highly specific fluorescent marker for the degenerating neurons. Consistent with ZP4 staining, we found the beneficial effects of rhEpo on neuronal survival in hippocampus after TBI (n=5/group, p<0.05). Our results suggest that rhEpo can significantly reduce the pathological Zn(2+) accumulation in rat hippocampus after TBI as well as zinc-induced cell death in cultured cells, which may potentially contribute to its neuronal protection after excitotoxic brain damage. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 资助信息 | Jinling Hospital of Nanjing, Jiangsu Province, China[Q2008033] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1641]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Zhu, Lin,Wang, Han-dong,Yu, Xin-guang,et al. Erythropoietin prevents zinc accumulation and neuronal death after traumatic brain injury in rat hippocampus: In vitro and in vivo studies[J]. BRAIN RESEARCH,2009,1289:96-105. |
| APA | Zhu, Lin.,Wang, Han-dong.,Yu, Xin-guang.,Jin, Wei.,Qiao, Liang.,...&Zhou, Jing.(2009).Erythropoietin prevents zinc accumulation and neuronal death after traumatic brain injury in rat hippocampus: In vitro and in vivo studies.BRAIN RESEARCH,1289,96-105. |
| MLA | Zhu, Lin,et al."Erythropoietin prevents zinc accumulation and neuronal death after traumatic brain injury in rat hippocampus: In vitro and in vivo studies".BRAIN RESEARCH 1289(2009):96-105. |
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来源：上海神经科学研究所
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