Inherent Dynamics of the Acid-Sensing Ion Channel 1 Correlates with the Gating Mechanism
文献类型：期刊论文
| 作者 | Yang, Huaiyu ; Yu, Ye ; Li, Wei-Guang ; Yu, Fang ; Cao, Hui ; Xu, Tian-Le(徐天乐) ; Jiang, Hualiang |
| 刊名 | PLOS BIOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 7期号:7页码:e1000151-e1000152 |
| 关键词 | NORMAL-MODE ANALYSIS NICOTINIC ACETYLCHOLINE-RECEPTOR EPITHELIAL NA+ CHANNEL SINGLE-PARAMETER PROTEINS CONTRIBUTES FLEXIBILITY SELECTIVITY PERMEATION MOTIONS |
| ISSN号 | 1544-9173 |
| 通讯作者 | Yang, HY (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, State Key Lab Drug Res, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn |
| 英文摘要 | The acid-sensing ion channel 1 (ASIC1) is a key receptor for extracellular protons. Although numerous structural and functional studies have been performed on this channel, the structural dynamics underlying the gating mechanism remains unknown. We used normal mode analysis, mutagenesis, and electrophysiological methods to explore the relationship between the inherent dynamics of ASIC1 and its gating mechanism. Here we show that a series of collective motions among the domains and subdomains of ASIC1 correlate with its acid-sensing function. The normal mode analysis result reveals that the intrinsic rotation of the extracellular domain and the collective motions between the thumb and finger induced by proton binding drive the receptor to experience a deformation from the extracellular domain to the transmembrane domain, triggering the channel pore to undergo "twist-to-open" motions. The movements in the transmembrane domain indicate that the likely position of the channel gate is around Leu440. These motion modes are compatible with a wide body of our complementary mutations and electrophysiological data. This study provides the dynamic fundamentals of ASIC1 gating. A |
| 学科主题 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
| 收录类别 | SCI |
| 资助信息 | State Key Program of Basic Research of China[2009CB918502, 2006CB500803]; China Postdoctoral Science Foundation[20080440095]; National Natural Science Foundation of China[20721003, 20720102040, 30830035, 30700145, 30621062] |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1656]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Yang, Huaiyu,Yu, Ye,Li, Wei-Guang,et al. Inherent Dynamics of the Acid-Sensing Ion Channel 1 Correlates with the Gating Mechanism[J]. PLOS BIOLOGY,2009,7(7):e1000151-e1000152. |
| APA | Yang, Huaiyu.,Yu, Ye.,Li, Wei-Guang.,Yu, Fang.,Cao, Hui.,...&Jiang, Hualiang.(2009).Inherent Dynamics of the Acid-Sensing Ion Channel 1 Correlates with the Gating Mechanism.PLOS BIOLOGY,7(7),e1000151-e1000152. |
| MLA | Yang, Huaiyu,et al."Inherent Dynamics of the Acid-Sensing Ion Channel 1 Correlates with the Gating Mechanism".PLOS BIOLOGY 7.7(2009):e1000151-e1000152. |
入库方式： OAI收割
来源：上海神经科学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。