RPAP3 Interacts With Reptin to Regulate UV-Induced Phosphorylation of H2AX and DNA Damage
文献类型：期刊论文
| 作者 | Ni, Lin ; Saeki, Makio ; Xu, Li ; Nakahara, Hirokazu ; Saijo, Masafumi ; Tanaka, Kiyoji ; Kamisaki, Yoshinori |
| 刊名 | JOURNAL OF CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2009 |
| 卷号 | 106期号:5页码:920-928 |
| 关键词 | RPAP3 MONAD REPTIN H2AX UV CHROMATIN REMODELING DOUBLE-STRAND BREAKS CHROMATIN-REMODELING COMPLEX CANCER METASTASIS BINDING-PROTEIN PONTIN TRANSCRIPTION CHECKPOINTS ACTIVATION HELICASE GROWTH |
| ISSN号 | 0730-2312 |
| 通讯作者 | Saeki, M (reprint author), Osaka Univ, Grad Sch Dent, Dept Pharmacol, 1-8 Yamadaoka, Suita, Osaka 5650871, Japan,msaeki@dent.osaka-u.ac.jp |
| 英文摘要 | We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-a and cycloheximide. By affinity purification and mass spectrometry, RNA polymerase II-associated protein 3 (RPAP3) was identified as a Monad binding protein and may function with Monad as a novel modulator of apoptosis pathways. Here we report that Reptin, a highly conserved AAA + ATPase that is part of various chromatin-remodeling complexes, is also involved in the association of RPAP3 by immunoprecipitation and confocal microscopic analysis. Overexpression of RPAP3 induced HEK293 cells to death after UV-irradiation. Loss of RPAP3 by RNAi improved HeLa cell survival after UV-induced DNA damage and attenuated the phosphorylation of H2AX. Depletion of Reptin reduced cell survival and facilitated the phosphorylation on H2AX. These results suggest that RPAP3 modulates UV-induced DNA damage by regulating H2AX phosphorylation. J. Cell. Biochem. 106: 920-928, 2009. (C) 2009 Wiley-Liss, Inc. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| 收录类别 | SCI |
| 资助信息 | Japanese Society for the Promotion of Science[20592172, 20390471]; Osaka Medical Research Foundation; Shanghai Municipal Education Commission |
| 语种 | 英语 |
| 公开日期 | 2012-07-13 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1666]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Ni, Lin,Saeki, Makio,Xu, Li,et al. RPAP3 Interacts With Reptin to Regulate UV-Induced Phosphorylation of H2AX and DNA Damage[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2009,106(5):920-928. |
| APA | Ni, Lin.,Saeki, Makio.,Xu, Li.,Nakahara, Hirokazu.,Saijo, Masafumi.,...&Kamisaki, Yoshinori.(2009).RPAP3 Interacts With Reptin to Regulate UV-Induced Phosphorylation of H2AX and DNA Damage.JOURNAL OF CELLULAR BIOCHEMISTRY,106(5),920-928. |
| MLA | Ni, Lin,et al."RPAP3 Interacts With Reptin to Regulate UV-Induced Phosphorylation of H2AX and DNA Damage".JOURNAL OF CELLULAR BIOCHEMISTRY 106.5(2009):920-928. |
入库方式： OAI收割
来源：上海神经科学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。