Actin Filament Assembly by Myristoylated, Alanine-rich C Kinase Substrate-Phosphatidylinositol-4,5-diphosphate Signaling Is Critical for Dendrite Branching
文献类型：期刊论文
| 作者 | Li, Haimin ; Chen, Gang ; Zhou, Bing ; Duan, Shumin |
| 刊名 | MOLECULAR BIOLOGY OF THE CELL
 |
| 出版日期 | 2008 |
| 卷号 | 19期号:11页码:4804-4813 |
| 关键词 | RAT-BRAIN HIPPOCAMPAL-NEURONS MARCKS SUBSTRATE PROTEIN DYNAMICS ADHESION PIP2 ELECTROPORATION MORPHOGENESIS |
| ISSN号 | 1059-1524 |
| 通讯作者 | Duan, S (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China,shumin@ion.ac.cn |
| 英文摘要 | Dendrites undergo extensive growth and branching at early stages, but relatively little is known about the molecular mechanisms underlying these processes. Here, we show that increasing the level of myristoylated, alanine-rich C kinase substrate ( MARCKS), a prominent substrate of protein kinase C and a phosphatidylinositol-4,5-diphosphate [PI(4,5)P2] sequestration protein highly expressed in the brain, enhanced branching and growth of dendrites both in vitro and in vivo. Conversely, knockdown of endogenous MARCKS by RNA interference reduced dendritic arborization. Results from expression of different mutants indicated that membrane binding is essential for MARCKS-induced dendritic morphogenesis. Furthermore, MARCKS increased the number and length of filamentous actin-based filopodia along neurites, as well as the motility of filopodia, in a PI(4,5)P2-dependent manner. Time-lapse imaging showed that MARCKS increased frequency of filopodia initiation but did not affect filopodia longevity, suggesting that MARCKS may increase dendritic branching through its action on filopodia initiation. These findings demonstrate a critical role for MARCKS PI( 4,5) P2 signaling in regulating dendrite development. |
| 学科主题 | Cell Biology |
| 收录类别 | SCI |
| 资助信息 | Major State Basic Research Program of China[2006CB806600]; National Natural Science Foundation of China[30730037]; Chinese Academic Sciences[KSCX2-YW-R-101]; Science and Technology Commission of Shanghai Municipality[07XD14039] |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1695]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Li, Haimin,Chen, Gang,Zhou, Bing,et al. Actin Filament Assembly by Myristoylated, Alanine-rich C Kinase Substrate-Phosphatidylinositol-4,5-diphosphate Signaling Is Critical for Dendrite Branching[J]. MOLECULAR BIOLOGY OF THE CELL,2008,19(11):4804-4813. |
| APA | Li, Haimin,Chen, Gang,Zhou, Bing,&Duan, Shumin.(2008).Actin Filament Assembly by Myristoylated, Alanine-rich C Kinase Substrate-Phosphatidylinositol-4,5-diphosphate Signaling Is Critical for Dendrite Branching.MOLECULAR BIOLOGY OF THE CELL,19(11),4804-4813. |
| MLA | Li, Haimin,et al."Actin Filament Assembly by Myristoylated, Alanine-rich C Kinase Substrate-Phosphatidylinositol-4,5-diphosphate Signaling Is Critical for Dendrite Branching".MOLECULAR BIOLOGY OF THE CELL 19.11(2008):4804-4813. |
入库方式： OAI收割
来源：上海神经科学研究所
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