A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons
文献类型：期刊论文
| 作者 | Wang, Zuoren
|
| 刊名 | EUROPEAN JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2007 |
| 卷号 | 26期号:9页码:2405-2416 |
| 关键词 | dopaminergic neuron giant gustatory neuron nociception painless MUSHROOM BODY SYNAPTIC-TRANSMISSION TASTE PERCEPTION ADULT DROSOPHILA EXCITABLE CELLS OPTICAL CONTROL MELANOGASTER ACTIVATION FIBER GENE |
| ISSN号 | 0953-816X |
| 通讯作者 | Zhang, W (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China,ZuorenWang@ion.ac.cn |
| 英文摘要 | In order to study the function of specific neural circuits, we generated UAS-Channelrhodopsin2 (ChR2) transgenic Drosophila and established a ChR2-based system that enables specific activation of targeted neurons in larval and adult fruit flies with blue light illumination, under the control of a newly designed light source that provides fully programmable stimulation patterns. We showed that stimulating selectively the nociceptor of larvae expressing ChR2 elicited light-induced 'pain' response, confined freely behaving larvae in defined area and directed larva migration along a preset route. In freely behaving adult flies, rapid photoactivation of targeted gustatory sensory neurons, dopaminergic modulatory neurons and motor neurons triggered the proboscis extension response, escaping reflex and changes in the locomotion pattern, respectively, with precise temporal control. This non-invasive method for remote control of animal behaviors also provides a potential tool for conducting 'gain of function' studies toward understanding how animal behaviors are controlled by neural activity. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1749]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_神经环路与动物行为研究组 |
| 推荐引用方式 GB/T 7714 | Wang, Zuoren. A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2007,26(9):2405-2416. |
| APA | Wang, Zuoren.(2007).A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons.EUROPEAN JOURNAL OF NEUROSCIENCE,26(9),2405-2416. |
| MLA | Wang, Zuoren."A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons".EUROPEAN JOURNAL OF NEUROSCIENCE 26.9(2007):2405-2416. |
入库方式： OAI收割
来源：上海神经科学研究所
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