Regulated ATP release from astrocytes through lysosome exocytosis
文献类型：期刊论文
| 作者 | Wang, Wei
|
| 刊名 | NATURE CELL BIOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 9期号:8页码:945-U115 |
| 关键词 | KISS-AND-RUN HIPPOCAMPAL SYNAPSES MULTIDRUG-RESISTANCE SECRETORY LYSOSOMES CALCIUM WAVES CELLS VESICLES RECEPTORS RELEVANCE PROTEINS |
| ISSN号 | 1465-7392 |
| 通讯作者 | Duan, SM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China,shumin@ion.ac.cn |
| 英文摘要 | Release of ATP from astrocytes is required for Ca2+ wave propagation among astrocytes(1-3) and for feedback modulation of synaptic functions(2,4,5). However, the mechanism of ATP release and the source of ATP in astrocytes are still not known. Here we show that incubation of astrocytes with FM dyes leads to selective labelling of lysosomes. Time-lapse confocal imaging of FM dye-labelled fluorescent puncta, together with extracellular quenching and total-internal-reflection fluorescence microscopy (TIRFM), demonstrated directly that extracellular ATP or glutamate induced partial exocytosis of lysosomes, whereas an ischaemic insult with potassium cyanide induced both partial and full exocytosis of these organelles. We found that lysosomes contain abundant ATP, which could be released in a stimulus-dependent manner. Selective lysis of lysosomes abolished both ATP release and Ca2+ wave propagation among astrocytes, implicating physiological and pathological functions of regulated lysosome exocytosis in these cells. |
| 学科主题 | Cell Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1757]  |
| 专题 | 上海神经科学研究所_神经所(总) 上海神经科学研究所_视知觉机制研究组 |
| 推荐引用方式 GB/T 7714 | Wang, Wei. Regulated ATP release from astrocytes through lysosome exocytosis[J]. NATURE CELL BIOLOGY,2007,9(8):945-U115. |
| APA | Wang, Wei.(2007).Regulated ATP release from astrocytes through lysosome exocytosis.NATURE CELL BIOLOGY,9(8),945-U115. |
| MLA | Wang, Wei."Regulated ATP release from astrocytes through lysosome exocytosis".NATURE CELL BIOLOGY 9.8(2007):945-U115. |
入库方式： OAI收割
来源：上海神经科学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。