Modulation of acid-sensing ion channels by Cu2+ in cultured hypothalamic neurons of the rat
文献类型：期刊论文
| 作者 | Wang, W. ; Yu, Y. ; Xu, T. -L. |
| 刊名 | NEUROSCIENCE
 |
| 出版日期 | 2007 |
| 卷号 | 145期号:2页码:631-641 |
| 关键词 | acid-sensing ion channels Cu2+ hypothalamus patch clamp membrane excitability HIPPOCAMPAL-NEURONS SENSORY NEURONS ACTIVATED CURRENTS GATED CHANNELS HIGH-AFFINITY TRACE COPPER SPINAL-CORD BRAIN CONTRIBUTES ISCHEMIA |
| ISSN号 | 0306-4522 |
| 通讯作者 | Xu, TL (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn |
| 英文摘要 | Acid-sensing ion channels (ASICs) are known to distribute throughout the nervous system and serve important roles in various physiological and pathological processes. However, the properties of ASICs in the hypothalamus, an important region of diencephalon, are little known. We herein used whole-cell patch-clamp recordings to characterize proton-induced cation currents in cultured hypothalamic neurons of the rat, and attributed these transient inward currents to ASICs based on their electrophysiological and pharmacological properties. We further examined the effects of Cu2+, the third most abundant trace element, on ASICs in hypothalamic neurons. Our results showed that this divalent cation reversibly and concentration-dependently inhibited the amplitude of ASIC currents, and slowed down the desensitization of ASIC channels. Our results also displayed that Cu2+ modulated ASICs independent of change in membrane potential and extracellular protons, suggesting a noncompetitive mechanism. Furthermore, micromolar concentration of Cu2+ attenuated the acid-induced membrane depolarization. Taken together, our data demonstrate a modulatory effect of Cu2+ on ASICs in native hypothalamic neurons and suggest a role of this endogenous metal ion in negatively modulating the increased neuronal membrane excitability caused by activation of ASICs. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2012-07-23 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/1782]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Wang, W.,Yu, Y.,Xu, T. -L.. Modulation of acid-sensing ion channels by Cu2+ in cultured hypothalamic neurons of the rat[J]. NEUROSCIENCE,2007,145(2):631-641. |
| APA | Wang, W.,Yu, Y.,&Xu, T. -L..(2007).Modulation of acid-sensing ion channels by Cu2+ in cultured hypothalamic neurons of the rat.NEUROSCIENCE,145(2),631-641. |
| MLA | Wang, W.,et al."Modulation of acid-sensing ion channels by Cu2+ in cultured hypothalamic neurons of the rat".NEUROSCIENCE 145.2(2007):631-641. |
入库方式： OAI收割
来源：上海神经科学研究所
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